Back to results
US 11,535,884 B2Granted
Methods and kits for targeted enrichment of target DNA with high GC content
AssigneeBerry Genomics Co., Ltd.
InventorsZiwei Qu, Tao Yu, Fangming Wang, Nannan Zhang, Jianguang Zhang
Life of this patent
Estimating the term…
Abstract
The present invention relates to a method for enrichment of target DNA with high GC content based on target sequence capture and multiple displacement amplification, as well as a kit suitable for this method. The present invention also relates to a method for constructing a sequencing library of target DNA with high GC content based on the enrichment method of the present invention.
Claims14
A method for enrichment of target DNA with high GC content, comprising the following steps:
- (a) designing single-stranded oligonucleotide probes against specific flanking sequences of the target DNA with high GC content, and capturing the target DNA from genome DNA by target sequence capture technology;
- (b) amplifying the captured target DNA with high GC content using multiple displacement amplification so as to obtain an amplification product;
- (c) subjecting the amplification product to enzyme digestion to remove branched DNA intermediates, so as to obtain a digested product;
- (d) purifying a DNA fragment with a length equal to or larger than 2 kb in the digested product, so as to obtain enriched target DNA with high GC content.
1 more element
A kit for enrichment of target DNA with high GC content, comprising
- single-stranded oligonucleotide probes against specific flanking sequences of the target DNA with high GC content, wherein the single-stranded oligonucleotide probes carry a biotin label,
- reagents for multiple displacement amplification,
- reagents for removing branched DNA intermediates by enzyme digestion, and
- reagents for purifying DNA fragments with a length equal to or larger than 2 kb.
1 more element
Dependencies are inferred from the claim text.