Microorganisms and methods for enhancing the availability of reducing equivalents in the presence of methanol, and for producing succinate related thereto

A non-naturally occurring microbial organism comprising:

• (a) a methanol metabolic pathway (MMP), wherein said non-naturally occurring microbial organism comprises at least one exogenous nucleic acid encoding a MMP enzyme expressed in a sufficient amount for biosynthesis of succinate, and wherein said MMP comprises:
• (i) a methanol dehydrogenase (EM9) from bacteria,
• (ii) an EM9 from bacteria and a formaldehyde activating enzyme (EM10), or
• (iii) a methanol methyltransferase (EM1) and a methylenetetrahydrofolate reductase (EM2), and
• wherein EM9 converts methanol to formaldehyde,
• wherein EM10 converts formaldehyde to methylene-tetrahydrofolate (THF),
• wherein EM1 converts methanol to methyl-THF, and
• wherein EM2 converts methyl-THF to methylene-THF;
• (b) a succinate pathway (SucP);
• and
• (c) a formaldehyde assimilation pathway (FAP), wherein said non-naturally occurring microbial organism comprises at least one exogenous nucleic acid encoding a FAP enzyme and wherein said FAP comprises:
• (i) a hexulose-6-phosphate synthase (EF1) and a 6-phospho-3-hexuloisomerase (EF2), or
• (ii) a dihydroxyacetone synthase (EF3) or a dihydroxyacetone kinase (EF4);
• wherein EF1 converts formaldehyde and D-ribulose-5-phosphate to hexulose-6-phosphate,
• wherein EF2 converts hexulose-6-phosphate to fructose-6-phosphate,
• wherein EF3 converts formaldehyde and xylulose-6-phosphate to dihydroxyacetone, and
• wherein EF4 converts dihydroxyacetone to dihydroxyacetone-phosphate;
• and
• wherein said non-naturally occurring microbial organism comprises an Escherichia coli or Saccharomyces cerevisiae.