DNA molecules producing custom designed replicating and non-replicating negative stranded RNA viruses and uses there of

A method for producing a non-replicating derivative of a Measles Virus (MV), the non-replicating derivatives being named as a virosome and the method comprising the following steps:

• a) co-transfecting a MV Packaging cell line with (i) a Cloning Plasmid and (ii) a Helper plasmid to obtain co-transfected cells, wherein the cloning plasmid comprises a MV genome-like replicon RNA coding for one or more non-MV genes and, optionally a subset of MV genes, and the helper plasmid codes for the N, P and L proteins of MV;
• wherein the MV packaging cell line may or may not be modified to express M or F or H proteins of MV stably, but not requiring the help of an exogenous vaccinia virus or an exogenous T7 RNA polymerase;
• and
• b) incubating the co-transfected cells at a temperature between 35° C. to 38° C. for 3 to 10 days in a culture medium to obtain non-replicating derivatives released into the culture medium, said non-replicating derivatives referred to as virosomes;
• wherein the non-replicating derivatives comprise a genome selected from the group consisting of:
• (a) coding exclusively non-MV genes;
• (b) coding for MV-N and MV-P genes along with non-MV genes;
• and (c) coding for MV-N, MV-P, and MV-L genes along with non-MV genes, wherein the non-replicating derivatives do not code for M, H, and F genes of Measles Virus.