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AssigneesArkansas State University—Jonesboro, Indiana University Research and Technology Corporation
InventorsLuis Fabricio Medina-Bolivar, Tianhong Yang, Keithanne Mockaitis
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The process and system led to the identification of prenyltransferase genes from elicitor-treated peanut hairy roots. One of the prenyltransferases, AhR4DT-1 catalyzes a key reaction involved in the biosynthesis of prenylated stilbenoids, in which resveratrol is prenylated at its C-4 position to form arachidin-2, while another, AhR3′DT-1, was able to add the prenyl group to C-3′ of resveratrol. Each of these prenyltransferases has a high specificity for stilbenoid substrates, and their subcellular location in the plastid was confirmed by fluorescence microscopy. Structure analysis of the prenylated stilbenoids suggest that these two prenyltransferase activities represent the first committed steps in the biosynthesis of a large number of prenylated stilbenoids and their derivatives in peanut.
A method of producing a prenylated stilbenoid in a transgenic organism, cell or tissue, the method comprising:
A method of producing a prenylated stilbenoid in a transgenic organism, cell or tissue, the method comprising:
A method for expressing a nucleic acid molecule that encodes a polypeptide having stilbenoid prenyltransferase activity in a transgenic organism, cell or tissue, the method comprising:
A method for expressing a polypeptide having stilbenoid prenyltransferase activity in a transgenic organism, cell or tissue, the method comprising:
A method of producing a prenylated stilbenoid in a transgenic organism, cell or tissue, the method comprising:
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