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US 11,535,837 B2Granted
Mannanase variants and polynucleotides encoding same
AssigneeNovozymes A/S
InventorsRakhi Saikia, Naveen Shivanand Raikar, Padmavathi Balumuri, Jens Erik Nielsen
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Abstract
The present invention relates to mannanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Claims13
An isolated mannanase variant, or recombinant polypeptide or an active fragment thereof comprising a modification at one or more positions corresponding to a position selected from the positions 101, 108, 132, 133, 177, 257 and 275 of the polypeptide of SEQ ID NO:
- 2, wherein each modification is independently a substitution, insertion, or deletion,
- wherein said variant, polypeptide or fragment thereof has at least 90% sequence identity but less than 100% sequence identity to the polypeptide of SEQ ID NO:
- 1 or the polypeptide of SEQ ID NO:
- 2,
- wherein said variant, polypeptide or fragment thereof has mannanase activity, and
- wherein said variant, polypeptide or fragment optionally further comprises a substitution, insertion, or deletion at one or more positions corresponding to a position selected from the positions 8, 9, 11, 13, 18, 21, 34, 37, 45, 47, 65, 100, 104, 107, 110, 114, 115, 116, 142, 147, 152, 154, 164, 169, 173, 174, 176, 180, 183, 185, 196, 199, 201, 202, 205, 206, 210, 215, 226, 229, 231, 239, 243, 245, 260, 267, 270, 278, 282, 283, 284, 288, 292, 293 and 295 of the polypeptide of SEQ ID NO:
- 2.
4 more elements
Dependencies are inferred from the claim text.