Variant G6P G7P glucoamylase compositions and methods

A composition comprising a variant glucoamylase enzyme, wherein the variant glucoamylase exhibits at least 95% identity to SEQ ID NO:15, wherein said variant glucoamylase enzyme has at least 1.1 fold better activity as compared to SEQ ID NO:25 under a condition selected from the group consisting of thermoactivity at about 30° C., thermoactivity at about 35° C., thermoactivity at about 40° C., thermoactivity at about 50° C., thermoactivity at about 60° C. and thermoactivity at about 70° C., wherein the variant glucoamylase enzyme consists of a catalytic domain;

• wherein the variant glucoamylase enzyme is not naturally occurring;
• and wherein an amino acid substitution(s) is selected from the group consisting of S188H/Y321W/Q419P, K5T/S188T, K5T/E68K/S188H, K5Y/S188H, K5Q/E68K/S188T, K5N/E68Y/S188T, K5H/S188H, K5S/E68Y/S188H, K5S/E68Y/S188T, K5Y/E68Y, K5S/E68S/S188T, K5H/E68S/S188H, K5H/E68K/S188T, K5P/S188H, K5Y/E68K, K5T/S188H, K5S/S188H, E68Y/S188H, E68Y/S188T, K5T/Y321F, K5Q/S188H/Q419P, K5P/E68Y/S188T/A422S, K5N/S188H/Q419P, Y321W/A422S, E68K/Q419P, E68S/S188T/Y321W/A422S, K5Q/Q419P/A422S, S188T/Y321F, S188T/Q419P/A422S, K5H/S188T/Q419P/A422S, K5H/A422S, S188H/Y321W, Q419P/A422S, K5N/A422S, E68Y/S188H/Y321F/Q419P, K5Q/S188H, E11S/Y55F/M141L/N168S/T388Q, E11S/T31N/Y55F/M141L/N168S/T388Q, L19I/A195V, M141L/N168S/Y227F/T362Q/T388Q/D440Q, E11S/M141L, E11S/L19I/144V/Y145A/T155A/D309S/L311V/Y412F/S472T, D309S/L311V, E11S/L19I/T31N/Y55F/A125P/N168S/Y227F, L19I/D309S/L311V/Y412F/A460S/S472T, E11S/L19I/N168S/Y227F/D309S/L311V/D440Q, L19I/Y412F, E11S/L19I/Y145A/A460S/S472T, E11S/M141L/N168S/D309S/L311V/T362Q/L364I/Y412F/A460S/S472T, E11S/Y55F/M141L/N168S/L311V/T362Q/L364I/A460S/S472T, E11S/L19I/Y55F/M141L/N168S/Y227F, L19I/T155A/A407S/Y412F/S472T, D53N/L149I/N150D/P186E/A317P, V42I/S103D/D106T/P186E/A317P/S434T/G478S, A317P/G478S, C26N/V42I/L149I/A317P/S355D/A374N, V42I/S103D/D106T/A317P, D53N/S434T, S103D/A317P/V342I, A256V/A317P, V42I/P186E, A317P/V342I/G478S, I224V/A256V/A317P/G478S, V42I/L149I/N150D/A317P/G478S, V42I/S103D/P186E/A317P/V342I/S355D/A374N/A391G, P186E/A317P/G478S, D53N/G446S, A256V/A317P/G478S, V42I/D53N/T57S/L149I/A317P/G478S, C26N/V42I/D53N/T57S/N150D/P186E/A317P/V342I/G478S, V42I/S355D, V42I/N150D/P186E/A317P, V42I/S434T/G478S, C26N/D53N/T57S/S103D/Q161P/A317P/G478S, V42I/A256V/A317P/S434T, V42I/S103D/A317P/A391G/G478S, E68Y/I69L/G178T/Y179F/S213T/H214C, E68Y/I69L/E85Q/N86Q/F394Y/V395L, E68Y/I69L, D118N/M119E, E68Y/I69L/V97I/T98S/A246S/S247G/T261G/A262S/L347N/E348I, E68Y/I69L/S452A/V453S, E68Y/I69L/S452A/V453S, E68Y/169L/E85Q/N86Q/L347N/E348I/Q418T/Q419P, E68Y/169L/E85Q/N86Q/S204N/T205A, A208T/K209R/E381N/S382D/Q418T/Q419P/A469S/A470T, E381N/S382D/Q418T/Q419P/T436S/E437A/A469S/A470T, Q418T/Q419P/A469S/A470T, V170I/A171T/E381N/S382D/Q418T/Q419P/A469S/A470T, F394Y/V395L/Q418T/Q419P/T436S/E437A/A469S/A470T, T78K/L79D/Q418T/Q419P/A469S/A470T, E68Y/I69L/V88I/T89S/T352S/V353I/Q399E/T400K/A469S/A470T, S72A/V73F/A153S/S306A/T476W, T370V/G475V, R49H/S72A/V73F/A92T/Y93K/A153S/S306A/T476W, E68Y/I69L/T219S/V220Q/A307E/S355D/L492P, E11S/V42I/L149I/I224V/A317P/T388Q/A460S/G478S, Y412F/Q418T/Q419P/A469S/A470T, L19I/D53N/I69L/V88I/S188H/A195V/Y321W/Y412F, V42I/D118N/M119E/T261A/L311V/Y321W/S355D, E11S/T57S/T89S, E11S/V42I/L79Y/D118N/M141L/L311V/A317P/Y321F, V42I/D53N/E68Y/A317P/L347N, I69L/S188H/A195V/A317P/Y321F/L364I/S434T, T57S/A195V/T261A/L311V/Y321F/L347N/E348I/S434T, E11S/V42I/T57S/E68Y/S188H/A195V/T261A, T57S/N168S/T261A/A317P/Y321W/V395L/Y412F, E11S/D53N/E68Y/D118N/S188H/A195V/Y412F/Q419P, E11S/V42I/D118N/M141L/N168S/Y321W/Y412F, E11S/D53N/T57S/E68Y/T89S/E381N/Y412F/Q419P/S434T, E11S/D53N/T57S/V88I/T89S/S188H/A195V/L311V/L347N/Y412F, A317P/Y412F, I69L/T89S/L311V/A317P/Y321F/Y412F/S434T, V42I/D53N/T57S/E68Y/V88I/Y412F, E11S/T57S/V88I/M141L/T261A/Y321W/L364I/Y412F, V42I/169L/S188H/A317P/Y321W/Y412F, E11S/V42I/D53N/T57S/L79Y/T89S/L311V/Y321W/Y412F, L19I/M141L/N168S/S188H/A195V/L311V/S355D/S434T, S188H/A195V/T261A/L311V/Y321F/L347N/E348I, E11S/L19I/D53N/T57S/N168S/L347N, E11S/169L/M119E/A195V/T261A/A317P/Y321W/L347N/Y412F, L79Y/V88I/T261A/A317P/Y321F/L364I/Y412F, E11S/V42I/E68Y/T89S/L311V/Y321F/T388Q/F394Y/V395L/Q419P, D53N/E68Y/V88I/M141L/N168S/Y321W/Y412F, E11S/I69L/L311V/A317P/Y321F/L347N/Y412F, D53N/T57S/M141L, D53N/V88I/T89S/D118N/E348I/S355D, E11S/S188H/A195V/A317P/L347N/E348I/Y412F/Q419P/S434T, E11S/D53N/169L/V88I/A317P/Q419P, E68Y/I69L/V88I/N168S/L364I/Q419P, E11S/V42I/V88I/T261A/Y321F/E348I/S355D, E11S/V42I/T57S/I69L/A195V/L347N/E348I/S355D, L19I/T89S/A317P/Y321F/S434T, E11S/V42I/L79Y/L311V/A317P/Y321W/L347N/E348I/L364I/Y412F, D53N/T57S/L347N/E348I/Y412F, D53N/T57S/E68Y/L79Y/Y412F/S434T, L19I/V88I/T89S/A317P, M119E/M141L/N168S/L311V/Y321F, E11S/T89S/Y321W, E11S/M141L/T261A/A317P/Y412F/S434T, V42I/L79Y/S188H/A195V/L311V/Y321F/S355D, V42I/E68Y/V88I/T261A/Y321W/L347N/E348I, L19I/E68Y/169L/M141L/Y321F/L347N/S355D/E381N/Y412F, V42I/D53N/I69L/L79Y/D118N/T261A/L311V/A317P/Y321F/L347N/Y412F, E68Y/S355D/L364I/S382D/T388Q/Q419P, E11S/L19I/V42I, E68Y/169L/D118N/M141L/Y321W/L347N,/E11S/V42I/E68Y/M141L/A317P/Y321F/E348I/S355D, E11S/V42I/E68Y/I69L/V88I/T89S/D118N/M119E/S188H/A195V/A317P/L347N/Y412F/Q419P, V42I/V88I/L311V/A317P/Y321F/L347N/E348I/T388Q/V395L/S434T, T261A/A317P/Y321W/E348I, E11S/V42I/E68Y/169L/D118N/M119E/Y412F, E11S/V42I/T57S/169L/L311V, V42I/Y412F, E11S/L19I/E68Y/M119E/N168S/Y412F/Q419P, L19I/L311V/Y412F, E11S/V42I/M119E/Y412F, E11S/L19I/V42I/D53N/S188H/A195V/L311V/Y321F/Y412F, E11S/L19I/V42I/T57S/D118N/N168S/L311V/Y412F, L19I/V42I/D53N/T57S/N168S/A317P/Y321W/Y412F, D53N/T57S/E68Y/N168S/S188H/A195V/L311V/Y321W/Y412F/Q419P, L19I/D53N/T57S/L311V/Y321W/Y412F, E11S/T57S/Y321W/Y412F/Q419P, D53N/S188H/A195V/L311V/Y412F, V42I/D53N/S188H/A195V/L311V/Y321F/Y412F, L19I/M119E/N168S/S188H/A195V/L311V/Y412F/Q419P, E11S/V42I/S188H/A195V/L311V/A317P/Y321W/Y412F/Q419P, E11S/V42I/M119E/N168S/L311V/Y412F, E11S/V42I/N168S/L311V/A317P/Y321W/Y412F/Q419P, E11S/V42I/D53N/E68Y/S188H/A195V/L311V/A317P/Y321F/Y412F, E11S/L19I/D53N/N168S/A195V/Y412F/Q419P, E11S/L19I/V42I/L311V/Y412F, V42I/D53N/N168S/L311V/Y412F, E11S/L311V/Y412F, E11S/D53N/T57S/L311V/Y321W/Y412F, E11S/L311V/Y321W/Y412F, E11S/V42I/L19I/D118N/N168S/L311V/A317P/Y412F/Q419P, E11S/L19I/V42I/E68Y/Y412F/Q419P, E11S/L19I/D53N/L311V, E11S/L19I/V42I/L311V/Y412F/Q419P, L19I/V42I/D53N/T57S/I69L/N168S/S188H/A195V/L311V/A317P/Y321W, E11S/L19I/V42I/T57S/L311V/A317P/Y321F/Y412F, L19I/V42I/169L/D118N/N168S/L311V/Y321W/Y412F/Q419P, E11S/V42I/D53N/E68Y/S188H/A195V/Y412F, E11S/L19I/V42I/D53N/T57S/N168S/L311V/Y321W/Y412F, E11S/V42I/L311V/Y412F, E11S/L19I/V42I/N168S/A195V/L311V/Y321F/Y412F, E11S/V42I/S188H/L311V/Y412F, L19I/V42I/T57S/E68Y/M119E/S188H/A195V/L311V/Y321F/Y412F, E11S/L19I/V42I/D53N/N168S/S188H/A195V, E11S/L19I/V42I/T57S/E68Y/A195V/L311V/Y321W, and E11S/L19I/V42I.

A composition comprising a variant glucoamylase enzyme comprising at least one amino acid substitution as compared to SEQ ID NO:15, wherein said amino acid substitution is at a position number selected from the group consisting of 5, 27, 36, 38, 39, 46, 47, 50, 65, 68, 79, 89, 98, 108, 121, 122, 124, 158, 161, 188, 192, 212, 215, 258, 262, 264, 298, 321, 400, 412, 419, 420, 422, 423, 457, 491, 497, 498, 503, 508, 509, 510, 7, 9, 11, 19, 26, 31, 42, 44, 49, 53, 55, 57, 69, 72, 73, 78, 85, 86, 88, 92, 93, 94, 97, 103, 106, 118, 119, 125, 141, 145, 149, 150, 153, 155, 168, 170, 171, 178, 179, 186, 195, 204, 205, 208, 209, 213, 214, 219, 220, 224, 227, 246, 247, 256, 261, 306, 307, 309, 311, 317, 342, 347, 348, 352, 353, 355, 362, 364, 370, 374, 381, 382, 388, 391, 394, 395, 399, 407, 418, 434, 436, 437, 440, 446, 452, 453, 460, 469, 470, 472, 475, 476, 478 and 492, wherein said variant enzyme is at least 95% identical to SEQ ID NO:15, wherein the variant glucoamylase enzyme does not include a starch binding domain;

• wherein the variant glucoamylase enzyme is not naturally occurring;
• and wherein said amino acid substitution(s) is selected from the group consisting of S188H/Y321W/Q419P, K5T/S188T, K5T/E68K/S188H, K5Y/S188H, K5Q/E68K/S188T, K5N/E68Y/S188T, K5H/S188H, K5S/E68Y/S188H, K5S/E68Y/S188T, K5Y/E68Y, K5S/E68S/S188T, K5H/E68S/S188H, K5H/E68K/S188T, K5P/S188H, K5Y/E68K, K5T/S188H, K5S/S188H, E68Y/S188H, E68Y/S188T, K5T/Y321F, K5Q/S188H/Q419P, K5P/E68Y/S188T/A422S, K5N/S188H/Q419P, Y321W/A422S, E68K/Q419P, E68S/S188T/Y321W/A422S, K5Q/Q419P/A422S, S188T/Y321F, S188T/Q419P/A422S, K5H/S188T/Q419P/A422S, K5H/A422S, S188H/Y321W, Q419P/A422S, K5N/A422S, E68Y/S188H/Y321F/Q419P, K5Q/S188H, E11S/Y55F/M141L/N168S/T388Q, E11S/T31N/Y55F/M141L/N168S/T388Q, L19I/A195V, M141L/N168S/Y227F/T362Q/T388Q/D440Q, E11S/M141L, E11S/L19I/144V/Y145A/T155A/D309S/L311V/Y412F/S472T, D309S/L311V, E11S/L19I/T31N/Y55F/A125P/N168S/Y227F, L19I/D309S/L311V/Y412F/A460S/S472T, E11S/L19I/N168S/Y227F/D309S/L311V/D440Q, L19I/Y412F, E11S/L19I/Y145A/A460S/S472T, E11S/M141L/N168S/D309S/L311V/T362Q/L364I/Y412F/A460S/S472T, E11S/Y55F/M141L/N168S/L311V/T362Q/L364I/A460S/S472T, E11S/L19I/Y55F/M141L/N168S/Y227F, L19I/T155A/A407S/Y412F/S472T, D53N/L149I/N150D/P186E/A317P, V42I/S103D/D106T/P186E/A317P/S434T/G478S, A317P/G478S, C26N/V42I/L149I/A317P/S355D/A374N, V42I/S103D/D106T/A317P, D53N/S434T/S103D/A317P/V342I, A256V/A317P, V42I/P186E, A317P/V342I/G478S, I224V/A256V/A317P/G478S, V42I/L149I/N150D/A317P/G478S, V42I/S103D/P186E/A317P/V342I/S355D/A374N/A391G, P186E/A317P/G478S, D53N/G446S, A256V/A317P/G478S, V42I/D53N/T57S/L149I/A317P/G478S, C26N/V42I/D53N/T57S/N150D/P186E/A317P/V342I/G478S, V42I/S355D, V42I/N150D/P186E/A317P, V42I/S434T/G478S, C26N/D53N/T57S/S103D/Q161P/A317P/G478S, V42I/A256V/A317P/S434T, V42I/S103D/A317P/A391G/G478S, E68Y/169L/G178T/Y179F/S213T/H214C, E68Y/I69L/E85Q/N86Q/F394Y/V395L, E68Y/169L, D118N/M119E, E68Y/I69L/V97I/T98S/A246S/S247G/T261G/A262S/L347N/E348I, E68Y/I69L/S452A/V453S, E68Y/I69L/S452A/V453S, E68Y/I69L/E85Q/N86Q/L347N/E348I/Q418T/Q419P, E68Y/I69L/E85Q/N86Q/S204N/T205A, A208T/K209R/E381N/S382D/Q418T/Q419P/A469S/A470T, E381N/S382D/Q418T/Q419P/T436S/E437A/A469S/A470T, Q418T/Q419P/A469S/A470T, V170I/A171T/E381N/S382D/Q418T/Q419P/A469S/A470T, F394Y/V395L/Q418T/Q419P/T436S/E437A/A469S/A470T, T78K/L79D/Q418T/Q419P/A469S/A470T, E68Y/I69L/V88I/T89S/T352S/V353I/Q399E/T400K/A469S/A470T, S72A/V73F/A153S/S306A/T476W, T370V/G475V, R49H/S72A/V73F/A92T/Y93K/A153S/S306A/T476W, E68Y/I69L/T219S/V220Q/A307E/S355D/L492P, E11S/V42I/L149I/I224V/A317P/T388Q/A460S/G478S, Y412F/Q418T/Q419P/A469S/A470T/L19I/D53N/I69L/V88I/S188H/A195V/Y321W/Y412F, V42I/D118N/M119E/T261A/L311V/Y321W/S355D, E11S/T57S/T89S, E11S/V42I/L79Y/D118N/M141L/L311V/A317P/Y321F, V42I/D53N/E68Y/A317P/L347N, I69L/S188H/A195V/A317P/Y321F/L364I/S434T, T57S/A195V/T261A/L311V/Y321F/L347N/E348I/S434T, E11S/V42I/T57S/E68Y/S188H/A195V/T261A, T57S/N168S/T261A/A317P/Y321W/V395L/Y412F, E11S/D53N/E68Y/D118N/S188H/A195V/Y412F/Q419P, E11S/V42I/D118N/M141L/N168S/Y321W/Y412F, E11S/D53N/T57S/E68Y/T89S/E381N/Y412F/Q419P/S434T, E11S/D53N/T57S/V88I/T89S/S188H/A195V/L311V/L347N/Y412F, A317P/Y412F, I69L/T89S/L311V/A317P/Y321F/Y412F/S434T, V42I/D53N/T57S/E68Y/V88I/Y412F, E11S/T57S/V88I/M141L/T261A/Y321W/L364I/Y412F, V42I/I69L/S188H/A317P/Y321W/Y412F, E11S/V42I/D53N/T57S/L79Y/T89S/L311V/Y321W/Y412F, L19I/M141L/N168S/S188H/A195V/L311V/S355D/S434T, S188H/A195V/T261A/L311V/Y321F/L347N/E348I, E11S/L19I/D53N/T57S/N168S/L347N, E11S/I69L/M119E/A195V/T261A/A317P/Y321W/L347N/Y412F, L79Y/V88I/T261A/A317P/Y321F/L364I/Y412F, E11S/V42I/E68Y/T89S/L311V/Y321F/T388Q/F394Y/V395L/Q419P, D53N/E68Y/V88I/M141L/N168S/Y321W/Y412F, E11S/169L/L311V/A317P/Y321F/L347N/Y412F, D53N/T57S/M141L, D53N/V88I/T89S/D118N/E348I/S355D, E11S/S188H/A195V/A317P/L347N/E348I/Y412F/Q419P/S434T, E11S/D53N/I69L/V88I/A317P/Q419P, E68Y/I69L/V88I/N168S/L364I/Q419P, E11S/V42I/V88I/T261A/Y321F/E348I/S355D, E11S/V42I/T57S/I69L/A195V/L347N/E348I/S355D, L19I/T89S/A317P/Y321F/S434T, E11S/V42I/L79Y/L311V/A317P/Y321W/L347N/E348I/L364I/Y412F, D53N/T57S/L347N/E348I/Y412F, D53N/T57S/E68Y/L79Y/Y412F/S434T, L19I/V88I/T89S/A317P, M119E/M141L/N168S/L311V/Y321F, E11S/T89S/Y321W, E11S/M141L/T261A/A317P/Y412F/S434T, V42I/L79Y/S188H/A195V/L311V/Y321F/S355D, V42I/E68Y/V88I/T261A/Y321W/L347N/E348I, L19I/E68Y/I69L/M141L/Y321F/L347N/S355D/E381N/Y412F, V42I/D53N/I69L/L79Y/D118N/T261A/L311V/A317P/Y321F/L347N/Y412F, E68Y/S355D/L364I/S382D/T388Q/Q419P, E11S/L19I/V42I/E68Y/I69L/D118N/M141L/Y321W/L347N, E11S/V42I/E68Y/M141L/A317P/Y321F/E348I/S355D, E11S/V42I/E68Y/I69L/V88I/T89S/D118N/M119E/S188H/A195V/A317P/L347N/Y412F/Q419P, V42I/V88I/L311V/A317P/Y321F/L347N/E348I/T388Q/V395L/S434T, T261A/A317P/Y321W/E348I, E11S/V42I/E68Y/I69L/D118N/M119E/Y412F, E11S/V42I/T57S/I69L/L311V, V42I/Y412F, E11S/L19I/E68Y/M119E/N168S/Y412F/Q419P, L19I/L311V/Y412F, E11S/V42I/M119E/Y412F, E11S/L19I/V42I/D53N/S188H/A195V/L311V/Y321F/Y412F, E11S/L19I/V42I/T57S/D118N/N168S/L311V/Y412F, L19I/V42I/D53N/T57S/N168S/A317P/Y321W/Y412F, D53N/T57S/E68Y/N168S/S188H/A195V/L311V/Y321W/Y412F/Q419P, L19I/D53N/T57S/L311V/Y321W/Y412F, E11S/T57S/Y321W/Y412F/Q419P, D53N/S188H/A195V/L311V/Y412F, V42I/D53N/S188H/A195V/L311V/Y321F/Y412F, L19I/M119E/N168S/S188H/A195V/L311V/Y412F/Q419P, E11S/V42I/S188H/A195V/L311V/A317P/Y321W/Y412F/Q419P, E11S/V42I/M119E/N168S/L311V/Y412F, E11S/V42I/N168S/L311V/A317P/Y321W/Y412F/Q419P, E11S/V42I/D53N/E68Y/S188H/A195V/L311V/A317P/Y321F/Y412F, E11S/L19I/D53N/N168S/A195V/Y412F/Q419P, E11S/L19I/V42I/L311V/Y412F, V42I/D53N/N168S/L311V/Y412F, E11S/L311V/Y412F, E11S/D53N/T57S/L311V/Y321W/Y412F, E11S/L311V/Y321W/Y412F, E11S/V42I/I69L/D118N/N168S/L311V/A317P/Y412F/Q419P, E11S/L19I/V42I/E68Y/Y412F/Q419P, E11S/L19I/D53N/L311V, E11S/L19I/V42I/L311V/Y412F/Q419P, L19I/V42I/D53N/T57S/I69L/N168S/S188H/A195V/L311V/A317P/Y321W, E11S/L19I/V42I/T57S/L311V/A317P/Y321F/Y412F, L19I/V42I/I69L/D118N/N168S/L311V/Y321W/Y412F/Q419P, E11S/V42I/D53N/E68Y/S188H/A195V/Y412F, E11S/L19I/V42I/D53N/T57S/N168S/L311V/Y321W/Y412F, E11S/V42I/L311V/Y412F, E11S/L19I/V42I/N168S/A195V/L311V/Y321F/Y412F, E11S/V42I/S188H/L311V/Y412F, L19I/V42I/T57S/E68Y/M119E/S188H/A195V/L311V/Y321F/Y412F, E11S/L19I/V42I/D53N/N168S/S188H/A195V, E11S/L19I/V42I/T57S/E68Y/A195V/L311V/Y321W, and E11S/L19I/V42I.

A composition comprising a variant glucoamylase enzyme comprising at least one amino acid substitution as compared to SEQ ID NO:15, wherein said amino acid substitution is at a position number selected from the group consisting of 5, 27, 36, 38, 39, 46, 47, 50, 65, 68, 79, 89, 98, 108, 121, 122, 124, 158, 161, 188, 192, 212, 215, 258, 262, 264, 298, 321, 400, 412, 419, 420, 422, 423, 457, 491, 497, 498, 503, 508, 509, 510, 7, 9, 11, 19, 26, 31, 42, 44, 49, 53, 55, 57, 69, 72, 73, 78, 85, 86, 88, 92, 93, 94, 97, 103, 106, 118, 119, 125, 141, 145, 149, 150, 153, 155, 168, 170, 171, 178, 179, 186, 195, 204, 205, 208, 209, 213, 214, 219, 220, 224, 227, 246, 247, 256, 261, 306, 307, 309, 311, 317, 342, 347, 348, 352, 353, 355, 362, 364, 370, 374, 381, 382, 388, 391, 394, 395, 399, 407, 418, 434, 436, 437, 440, 446, 452, 453, 460, 469, 470, 472, 475, 476, 478 and 492, wherein said variant glucoamylase enzyme has at least 1.1 fold better activity as compared to SEQ ID NO:15 under a condition selected from the group consisting of thermoactivity at about 30° C., thermoactivity at about 35° C., thermoactivity at about 40° C., thermoactivity at about 50° C., thermoactivity at about 60° C. and thermoactivity at about 70° C.;

• wherein said variant enzyme is at least 95% identical to SEQ ID NO:15, wherein the variant glucoamylase enzyme does not include a starch binding domain;
• wherein the variant glucoamylase enzyme is not naturally occurring;
• and wherein said amino acid substitution(s) is selected from the group consisting of S188H/Y321W/Q419P, K5T/S188T, K5T/E68K/S188H, K5Y/S188H, K5Q/E68K/S188T, K5N/E68Y/S188T, K5H/S188H, K5S/E68Y/S188H, K5S/E68Y/S188T, K5Y/E68Y, K5S/E68S/S188T, K5H/E68S/S188H, K5H/E68K/S188T, K5P/S188H, K5Y/E68K, K5T/S188H, K5S/S188H, E68Y/S188H, E68Y/S188T, K5T/Y321F, K5Q/S188H/Q419P, K5P/E68Y/S188T/A422S, K5N/S188H/Q419P, Y321W/A422S, E68K/Q419P, E68S/S188T/Y321W/A422S, K5Q/Q419P/A422S, S188T/Y321F, S188T/Q419P/A422S, K5H/S188T/Q419P/A422S, K5H/A422S, S188H/Y321W, Q419P/A422S, K5N/A422S, E68Y/S188H/Y321F/Q419P, K5Q/S188H, E11S/Y55F/M141L/N168S/T388Q, E11S/T31N/Y55F/M141L/N168S/T388Q, L19I/A195V, M141L/N168S/Y227F/T362Q/T388Q/D440Q, E11S/M141L, E11S/L19I/144V/Y145A/T155A/D309S/L311V/Y412F/S472T, D309S/L311V, E11S/L19I/T31N/Y55F/A125P/N168S/Y227F, L19I/D309S/L311V/Y412F/A460S/S472T, E11S/L19I/N168S/Y227F/D309S/L311V/D440Q, L19I/Y412F, E11S/L19I/Y145A/A460S/S472T, E11S/M141L/N168S/D309S/L311V/T362Q/L364I/Y412F/A460S/S472T, E11S/Y55F/M141L/N168S/L311V/T362Q/L364I/A460S/S472T, E11S/L19I/Y55F/M141L/N168S/Y227F, L19I/T155A/A407S/Y412F/S472T, D53N/L149I/N150D/P186E/A317P, V42I/S103D/D106T/P186E/A317P/S434T/G478S, A317P/G478S, C26N/V42I/L149I/A317P/S355D/A374N, V42I/S103D/D106T/A317P, D53N/S434T/S103D/A317P/V342I, A256V/A317P, V42I/P186E, A317P/V342I/G478S, I224V/A256V/A317P/G478S, V42I/L149I/N150D/A317P/G478S, V42I/S103D/P186E/A317P/V342I/S355D/A374N/A391G, P186E/A317P/G478S, D53N/G446S, A256V/A317P/G478S, V42I/D53N/T57S/L149I/A317P/G478S, C26N/V42I/D53N/T57S/N150D/P186E/A317P/V342I/G478S, V42I/S355D, V42I/N150D/P186E/A317P, V42I/S434T/G478S, C26N/D53N/T57S/S103D/Q161P/A317P/G478S, V42I/A256V/A317P/S434T, V42I/S103D/A317P/A391G/G478S, E68Y/I69L/G178T/Y179F/S213T/H214C, E68Y/I69L/E85Q/N86Q/F394Y/V395L, E68Y/I69L, D118N/M119E, E68Y/I69L/V97I/T98S/A246S/S247G/T261G/A262S/L347N/E348I, E68Y/I69L/S452A/V453S, E68Y/I69L/S452A/V453S, E68Y/I69L/E85Q/N86Q/L347N/E348I/Q418T/Q419P, E68Y/I69L/E85Q/N86Q/S204N/T205A, A208T/K209R/E381N/S382D/Q418T/Q419P/A469S/A470T, E381N/S382D/Q418T/Q419P/T436S/E437A/A469S/A470T, Q418T/Q419P/A469S/A470T, V170I/A171T/E381N/S382D/Q418T/Q419P/A469S/A470T, F394Y/V395L/Q418T/Q419P/T436S/E437A/A469S/A470T, T78K/L79D/Q418T/Q419P/A469S/A470T, E68Y/I69L/V88I/T89S/T352S/V353I/Q399E/T400K/A469S/A470T, S72A/V73F/A153S/S306A/T476W, T370V/G475V, R49H/S72A/V73F/A92T/Y93K/A153S/S306A/T476W, E68Y/I69L/T219S/V220/A307E/S355D/L492P, E11S/V42I/L149I/I224V/A317P/T388Q/A460S/G478S, Y412F/Q418T/Q419P/A469S/A470T/L19I/D53N/I69L/V88I/S188H/A195V/Y321W/Y412F, V42I/D118N/M119E/T261A/L311V/Y321W/S355D, E11S/T57S/T89S, E11S/V42I/L79Y/D118N/M141L/L311V/A317P/Y321F, V42I/D53N/E68Y/A317P/L347N, I69L/S188H/A195V/A317P/Y321F/L364I/S434T, T57S/A195V/T261A/L311V/Y321F/L347N/E348I/S434T, E11S/V42I/T57S/E68Y/S188H/A195V/T261A, T57S/N168S/T261A/A317P/Y321W/V395L/Y412F, E11S/D53N/E68Y/D118N/S188H/A195V/Y412F/Q419P, E11S/V42I/D118N/M141L/N168S/Y321W/Y412F, E11S/D53N/T57S/E68Y/T89S/E381N/Y412F/Q419P/S434T, E11S/D53N/T57S/V88I/T89S/S188H/A195V/L311V/L347N/Y412F, A317P/Y412F, I69L/T89S/L311V/A317P/Y321F/Y412F/S434T, V42I/D53N/T57S/E68Y/V88I/Y412F, E11S/T57S/V88I/M141L/T261A/Y321W/L364I/Y412F, V42I/I69L/S188H/A317P/Y321W/Y412F, E11S/V42I/D53N/T57S/L79Y/T89S/L311V/Y321W/Y412F, L19I/M141L/N168S/S188H/A195V/L311V/S355D/S434T, S188H/A195V/T261A/L311V/Y321F/L347N/E348I, E11S/L19I/D53N/T57S/N168S/L347N, E11S/I69L/M119E/A195V/T261A/A317P/Y321W/L347N/Y412F, L79Y/V88I/T261A/A317P/Y321F/L364I/Y412F, E11S/V42I/E68Y/T89S/L311V/Y321F/T388Q/F394Y/V395L/Q419P, D53N/E68Y/V88I/M141L/N168S/Y321W/Y412F, E11S/I69L/L311V/A317P/Y321F/L347N/Y412F, D53N/T57S/M141L, D53N/V88I/T89S/D118N/E348I/S355D, E11S/S188H/A195V/A317P/L347N/E348I/Y412F/Q419P/S434T, E11S/D53N/I69L/V88I/A317P/Q419P, E68Y/I69L/V88I/N168S/L364I/Q419P, E11S/V42I/V88I/T261A/Y321F/E348I/S355D, E11S/V42I/T57S/I69L/A195V/L347N/E348I/S355D, L19I/T89S/A317P/Y321F/S434T, E11S/V42I/L79Y/L311V/A317P/Y321W/L347N/E348I/L364I/Y412F, D53N/T57S/L347N/E348I/Y412F, D53N/T57S/E68Y/L79Y/Y412F/S434T, L19I/V88I/T89S/A317P, M119E/M141L/N168S/L311V/Y321F, E11S/T89S/Y321W, E11S/M141L/T261A/A317P/Y412F/S434T, V42I/L79Y/S188H/A195V/L311V/Y321F/S355D, V42I/E68Y/V88I/T261A/Y321W/L347N/E348I, L19I/E68Y/I69L/M141L/Y321F/L347N/S355D/E381N/Y412F, V42I/D53N/I69L/L79Y/D118N/T261A/L311V/A317P/Y321F/L347N/Y412F, E68Y/S355D/L364I/S382D/T388Q/Q419P, E11S/L19I/V42I/E68Y/I69L/D118N/M141L/Y321W/L347N, E11S/V42I/E68Y/M141L/A317P/Y321F/E348I/S355D, E11S/V42I/E68Y/I69L/V88I/T89S/D118N/M119E/S188H/A195V/A317P/L347N/Y412F/Q419P, V42I/V88I/L311V/A317P/Y321F/L347N/E348I/T388Q/V395L/S434T, T261A/A317P/Y321W/E348I, E11S/V42I/E68Y/I69L/D118N/M119E/Y412F, E11S/V42I/T57S/I69L/L311V, V42I/Y412F, E11S/L19I/E68Y/M119E/N168S/Y412F/Q419P, L19I/L311V/Y412F, E11S/V42I/M119E/Y412F, E11S/L19I/V42I/D53N/S188H/A195V/L311V/Y321F/Y412F, E11S/L19I/V42I/T57S/D118N/N168S/L311V/Y412F, L19I/V42I/D53N/T57S/N168S/A317P/Y321W/Y412F, D53N/T57S/E68Y/N168S/S188H/A195V/L311V/Y321W/Y412F/Q419P, L19I/D53N/T57S/L311V/Y321W/Y412F, E11S/T57S/Y321W/Y412F/Q419P, D53N/S188H/A195V/L311V/Y412F, V42I/D53N/S188H/A195V/L311V/Y321F/Y412F, L19I/M119E/N168S/S188H/A195V/L311V/Y412F/Q419P, E11S/V42I/S188H/A195V/L311V/A317P/Y321W/Y412F/Q419P, E11S/V42I/M119E/N168S/L311V/Y412F, E11S/V42I/N168S/L311V/A317P/Y321W/Y412F/Q419P, E11S/V42I/D53N/E68Y/S188H/A195V/L311V/A317P/Y321F/Y412F, E11S/L19I/D53N/N168S/A195V/Y412F/Q419P, E11S/L19I/V42I/L311V/Y412F, V42I/D53N/N168S/L311V/Y412F, E11S/L311V/Y412F, E11S/D53N/T57S/L311V/Y321W/Y412F, E11S/L311V/Y321W/Y412F, E11S/V42I/I69L/D118N/N168S/L311V/A317P/Y412F/Q419P, E11S/L19I/V42I/E68Y/Y412F/Q419P, E11S/L19I/D53N/L311V, E11S/L19I/V42I/L311V/Y412F/Q419P, L19I/V42I/D53N/T57S/I69L/N168S/S188H/A195V/L311V/A317P/Y321W, E11S/L19I/V42I/T57S/L311V/A317P/Y321F/Y412F, L19I/V42I/I69L/D118N/N168S/L311V/Y321W/Y412F/Q419P, E11S/V42I/D53N/E68Y/S188H/A195V/Y412F, E11S/L19I/V42I/D53N/T57S/N168S/L311V/Y321W/Y412F, E11S/V42I/L311V/Y412F, E11S/L19I/V42I/N168S/A195V/L311V/Y321F/Y412F, E11S/V42I/S188H/L311V/Y412F, L19I/V42I/T57S/E68Y/M119E/S188H/A195V/L311V/Y321F/Y412F, E11S/L19I/V42I/D53N/N168S/S188H/A195V, E11S/L19I/V42I/T57S/E68Y/A195V/L311V/Y321W, and E11S/L19I/V42I.

A composition comprising a C-terminally truncated variant glucoamylase enzyme of SEQ ID NO:13, wherein said truncation position is at a position number selected from the group consisting of 493, 500, 502, 503, 506, 509, 510, 513, 514, 519, 520, 522, 524, 525, 544, 549, 550, 551, 556, 558, 560, 563, 564 and 569;

• and wherein amino acid substitutions are selected from the group consisting of S188H/Y321W/Q419P, K5T/S188T, K5T/E68K/S188H, K5Y/S188H, K5Q/E68K/S188T, K5N/E68Y/S188T, K5H/S188H, K5S/E68Y/S188H, K5S/E68Y/S188T, K5Y/E68Y, K5S/E68S/S188T, K5H/E68S/S188H, K5H/E68K/S188T, K5P/S188H, K5Y/E68K, K5T/S188H, K5S/S188H, E68Y/S188H, E68Y/S188T, K5T/Y321F, K5Q/S188H/Q419P, K5P/E68Y/S188T/A422S, K5N/S188H/Q419P, Y321W/A422S, E68K/Q419P, E68S/S188T/Y321W/A422S, K5Q/Q419P/A422S, S188T/Y321F, S188T/Q419P/A422S, K5H/S188T/Q419P/A422S, K5H/A422S, S188H/Y321W, Q419P/A422S, K5N/A422S, E68Y/S188H/Y321F/Q419P, K5Q/S188H, E11S/Y55F/M141L/N168S/T388Q, E11S/T31N/Y55F/M141L/N168S/T388Q, L19I/A195V, M141L/N168S/Y227F/T362Q/T388Q/D440Q, E11S/M141L, E11S/L19I/144V/Y145A/T155A/D309S/L311V/Y412F/S472T, D309S/L311V, E11S/L19I/T31N/Y55F/A125P/N168S/Y227F, L19I/D309S/L311V/Y412F/A460S/S472T, E11S/L19I/N168S/Y227F/D309S/L311V/D440Q, L19I/Y412F, E11S/L19I/Y145A/A460S/S472T, E11S/M141L/N165S/D309S/L311V/T362Q/L364I/Y412F/A460S/S472T E11S/Y55F/M141L/N168S/L311V/T362Q/L364I/A460S/S472T, E11S/L19I/Y55F/M141L/N168S/Y227F, L19I/T155A/A407S/Y412F/S472T, D53N/L149I/N150D/P186E/A317P, V42I/S103D/D106T/P186E/A317P/S434T/G478S, A317P/G478S, C26N/V42I/L149I/A317P/S355D/A374N, V42I/S103D/D106T/A317P, D53N/S434T/S103D/A317P/V342I, A256V/A317P, V42I/P186E, A317P/V342I/G478S, I224V/A256V/A317P/G478S, V42I/L149I/N150D/A317P/G478S, V42I/S103D/P186E/A317P/V342I/S355D/A374N/A391G, P186E/A317P/G478S, D53N/G446S, A256V/A317P/G478S, V42I/D53N/T57S/L149I/A317P/G478S, C26N/V42I/D53N/T57S/N150D/P186E/A317P/V342I/G478S, V42I/S355D, V42I/N150D/P186E/A317P, V42I/S434T/G478S, C26N/D53N/T57S/S103D/Q161P/A317P/G478S, V42I/A256V/A317P/S434T, V42I/S103D/A317P/A391G/G478S, E68Y/I69L/G178T/Y179F/S213T/H214C, E68Y/I69L/E85Q/N86Q/F394Y/V395L, E68Y/I69L, D118N/M119E, E68Y/I69L/V97I/T98S/A246S/S247G/T261G/A262S/L347N/E348I, E68Y/I69L/S452A/V453S, E68Y/I69L/S452A/V453S, E68Y/I69L/E85Q/N86Q/L347N/E348I/Q418T/Q419P, E68Y/I69L/E85Q/N86Q/S204N/T205A, A208T/K209R/E381N/S382D/Q418T/Q419P/A469S/A470T, E381N/S382D/Q418T/Q419P/T436S/E437A/A469S/A470T, Q418T/Q419P/A469S/A470T, V170I/A171T/E381N/S382D/Q418T/Q419P/A469S/A470T, F394Y/V395L/Q418T/Q419P/T436S/E437A/A469S/A470T, T78K/L79D/Q418T/Q419P/A469S/A470T, E68Y/I69L/V88I/T98S/T352S/V353I/Q399E/T400K/A469S/A470T, S72A/V73F/A153S/S306A/T476W, T370V/G475V, R49H/S72A/V73F/A92T/Y93K/A153S/S306A/T476W, E68Y/I69L/T219S/V220Q/A307E/S355D/L492P, E11S/V42I/L149I/I224V/A317P/T388Q/A460S/G478S, Y412F/Q418T/Q419P/A469S/A470T/, L19I/D53N/169L/V88I/S188H/A195V/Y321W/Y412F, V42I/D118N/M119E/T261A/L311V/Y321W/S355D, E11S/T57S/T89S, E11S/V42I/L79Y/D118N/M141L/L311V/A317P/Y321F, V42I/D53N/E68Y/A317P/L347N, I69L/S188H/A195V/A317P/Y321F/L364I/S434T, T57S/A195V/T261A/L311V/Y321F/L347N/E348I/S434T, E11S/V42I/T57S/E68Y/S188H/A195V/T261A, T57S/N168S/T261A/A317P/Y321W/V395L/Y412F, E11S/D53N/E68Y/D118N/S188H/A195V/Y412F/Q419P, E11S/V42I/D118N/M141L/N168S/Y321W/Y412F, E11S/D53N/T57S/E68Y/T89S/E381N/Y412F/Q419P/S434T, E11S/D53N/T57S/V88I/T89S/S188H/A195V/L311V/L347N/Y412F, A317P/Y412F, I69L/T89S/L311V/A317P/Y321F/Y412F/S434T, V42I/D53N/T57S/E68Y/V88I/Y412F, E11S/T57S/V88I/M141L/T261A/Y321W/L364I/Y412F, V42I/I69L/S188H/A317P/Y321W/Y412F, E11S/V42I/D53N/T57S/L79Y/T89S/L311V/Y321W/Y412F, L19I/M141L/N168S/S188H/A195V/L311V/S355D/S434T, S188H/A195V/T261A/L311V/Y321F/L347N/E348I, E11S/L19I/D53N/T57S/N168S/L347N, E11S/I69L/M119E/A195V/T261A/A317P/Y321W/L347N/Y412F, L79Y/V88I/T261A/A317P/Y321F/L364I/Y412F, E11S/V42I/E68Y/T89S/L311V/Y321F/T388Q/F394Y/V395L/Q419P, D53N/E68Y/V88I/M141L/N168S/Y321W/Y412F, E11S/I69L/L311V/A317P/Y321F/L347N/Y412F, D53N/T57S/M141L, D53N/V88I/T89S/D118N/E348I/S355D, E11S/S188H/A195V/A317P/L347N/E348I/Y412F/Q419P/S434T, E11S/D53N/I69L/V88I/A317P/Q419P, E68Y/I69L/V88I/N168S/L364I/Q419P, E11S/V42I/V88I/T261A/Y321F/E348I/S355D, E11S/V42I/T57S/I69L/A195V/L347N/E348I/S355D, L19I/T89S/A317P/Y321F/S434T, E11S/V42I/L79Y/L311V/A317P/Y321W/L347N/E348I/L364I/Y412F, D53N/T57S/L347N/E348I/Y412F, D53N/T57S/E68Y/L79Y/Y412F/S434T, L19I/V88I/T89S/A317P, M119E/M141L/N168S/L311V/Y321F, E11S/T89S/Y321W, E11S/M141L/T261A/A317P/Y412F/S434T, V42I/L79Y/S188H/A195V/L311V/Y321F/S355D, V42I/E68Y/V88I/T261A/Y321W/L347N/E348I, L19I/E68Y/I69L/M141L/Y321F/L347N/S355D/E381N/Y412F, V42I/D53N/I69L/L79Y/D118N/T261A/L311V/A317P/Y321F/L347N/Y412F, E68Y/S355D/L364I/S382D/T388Q/Q419P, E11S/L19I/V42I/E68Y/I69L/D118N/M141L/Y321W/L347N, E11S/V42I/E68Y/M141L/A317P/Y321F/E348I/S355D, E11S/V42I/E68Y/I69L/V88I/T89S/D118N/M119E/S188H/A195V/A317P/L347N/Y412F/Q419P, V42I/V88I/L311V/A317P/Y321F/L347N/E348I/T388Q/V395L/S434T, T261A/A317P/Y321W/E348I, E11S/V42I/E68Y/I69L/D118N/M119E/Y412F, E11S/V42I/T57S/I69L/L311V, V42I/Y412F, E11S/L19I/E68Y/M119E/N168S/Y412F/Q419P, L19I/L311V/Y412F, E11S/V42I/M119E/Y412F, E11S/L19I/V42I/D53N/S188H/A195V/L311V/Y321F/Y412F, E11S/L19I/V42I/T57S/D118N/N168S/L311V/Y412F, L19I/V42I/D53N/T57S/N168S/A317P/Y321W/Y412F, D53N/T57S/E68Y/N168S/S188H/A195V/L311V/Y321W/Y412F/Q419P, L19I/D53N/T57S/L311V/Y321W/Y412F, E11S/T57S/Y321W/Y412F/Q419P, D53N/S188H/A195V/L311V/Y412F, V42I/D53N/S188H/A195V/L311V/Y321F/Y412F, L19I/M119E/N168S/S188H/A195V/L311V/Y412F/Q419P, E11S/V42I/S188H/A195V/L311V/A317P/Y321W/Y412F/Q419P, E11S/V42I/M119E/N168S/L311V/Y412F, E11S/V42I/N168S/L311V/A317P/Y321W/Y412F/Q419P, E11S/V42I/D53N/E68Y/S188H/A195V/L311V/A317P/Y321F/Y412F, E11S/L19I/D53N/N168S/A195V/Y412F/Q419P, E11S/L19I/V42I/L311V/Y412F, V42I/D53N/N168S/L311V/Y412F, E11S/L311V/Y412F, E11S/D53N/T57S/L311V/Y321W/Y412F, E11S/L311V/Y321W/Y412F, E11S/V42I/I69L/D118N/N168S/L311V/A317P/Y412F/Q419P, E11S/L19I/V42I/E68Y/Y412F/Q419P, E11S/L19I/D53N/L311V, E11S/L19I/V42I/L311V/Y412F/Q419P, L19I/V42I/D53N/T575/I69L/N168S/S188H/A195V/L311V/A317P/Y321W, E11S/L19I/V42I/T57S/L311V/A317P/Y321F/Y412F, L19I/V42I/I69L/D118N/N168S/L311V/Y321W/Y412F/Q419P, E11S/V42I/D53N/E68Y/S188H/A195V/Y412F, E11S/L19I/V42I/D53N/T57S/N168S/L311V/Y321W/Y412F, E11S/V42I/L311V/Y412F, E11S/L19I/V42I/N168S/A195V/L311V/Y321F/Y412F, E11S/V42I/S188H/L311V/Y412F, L19I/V42I/T57S/E68Y/M119E/S188H/A195V/L311V/Y321F/Y412F, E11S/L19I/V42I/D53N/N168S/S188H/A195V, E11S/L19I/V42I/T57S/E68Y/A195V/L311V/Y321W, and E11S/L19I/V42I.

A composition comprising a variant glucoamylase enzyme comprising at least one amino acid substitution as compared to SEQ ID NO:13, wherein said amino acid substitution is at a position selected from the group consisting of 5, 27, 36, 38, 46, 47, 50, 65, 68, 79, 89, 98, 108, 122, 124, 158, 161, 188, 192, 212, 215, 258, 262, 264, 298, 321, 400, 412, 420, 422, 457, 491, 497, 498, 503, 508, 509, 510, 516, 518, 521, 522, 526, 533, 534, 535, 540, 542, 544, 545, 549, 550, 551, 555, 556, 557, 559, 563, 564, 569, 570, 581 584, 7, 9, 11, 19, 26, 31, 42, 53, 55, 57, 69, 72, 73, 78, 85, 86, 88, 92, 93, 94, 97, 103, 106, 118, 125, 141, 145, 149, 150, 153, 155, 168, 170, 171, 178, 179, 186, 195, 204, 205, 208, 209, 213, 214, 219, 220, 224, 227, 246, 247, 256, 261, 306, 307, 311, 317, 342, 347, 348, 352, 353, 355, 362, 364, 370, 374, 381, 382, 388, 391, 394, 395, 399, 407, 418, 434, 436, 437, 440, 446, 452, 453, 460, 469, 470, 472, 475, 476, 478 and 492, wherein said variant enzyme is at least 95% identical to SEQ ID NO:13, and wherein the variant glucoamylase enzyme is not naturally occurring;

• and wherein amino acid substitutions are selected from the group consisting of T98S/Q161P/F516L, T98S/Q161P/N570P, T98S/Q161P, T550D/F555S, V65I/Y412F/Y503S/S518I, Y412F/Y503S/I564T, Y412F/Y503E, Y412F/Y503E/S518I, V65I/Y412F/Y503S, V65I/Y503E, Y503D/S518I/I564N, Y503E/S518L, V65I/Y503E/S544Q, V65I/Y503S, Y503E/S544E, V65I/Y503E/S544E/I564N, V65I/Y412F/Y503E, Y503E/S518I/S544Q, V65I/Y503E/S518M, Y412F/S518L, Y503E/S544Q, V65I/Y412F/Y503E/S544E/I564T/G569I, Y412F/Y503S, Y503E/I564T, S549D/F555D/G559N, S549D/F555R, K508T/L526N/S549D/G559N, K508T/G559N, F555D/I556P/G559N, S549D/F555R/I556P/G559N, F555R/I556P, L526I/F555R/I556P, F555R/I556P/G559N, K508T/I556P/G559N, F555D/I556P, K508T/L526N/A534T/S549D/I556P, K508T/L526I/S549D/G559N, K508N/L526I/S549E/F555L/G559N, L526N/S549D/F555D/I556P, L526I/F555D/G559N, A38V/F555R, L498T/T542N/T581N/T584N, T542N/T581N/T584N, S535Y/T542N, T540N/T584N, T540N/T542N/T581N/T584N, L498T/T581N/T584N, L498T/T584N, L498T/T542N/T581N, L498T/T542N, S535Y/T540N/T542N/T581N, Y321F/A422S, K5H/Y321W, K5N/Y321F, K5P/S188H, E68S/S188H/A422S, K5H/S188H/Y321F/A422S, S188H/A422S, S188H/A422S/V545A, K5Q/A422S, K5S/E68K/Y321W, K5H/Y321F/A422S, K5Y/A422S, K5Y/S188H/A422S, K5T/A422S, K5T/Y321W, Y321W/A422S, K5P/Y321F, K5H/S188H/Y321F/A422S, E68K/A422S, Y321F/A422S, K5H/Y321F, K5T/E68Y/S188T, K5S/E68Y/S188H, K5N/S188H, K5T/E68S/S188T, K5H/E68Y/S188P, K5P/E68Y/S188T, K5T/E68K/S188H, K5P/E68K/S188T, K5H/E68Y/S188T, K5T/S188H, K5T/E68N/S188T, K5Y/E68Y/S188T, K5S/E68Y/S188T, K5Q/E68K/S188H, K5P/E68N/S188H, K5P/E68N/S188T, K5P/E68Y/S188H, K5Y/E68Y/S188H, K5S/S188T, K5H/E68N/S188H, K5P/S188H, K5H/S46T/E68K/S188H, K5H/E68K/S188T, K501E68Y/S188T, K5Y/E68N/S188H, L19I/V42I/E68Y/N168S/S188H/A195V/Y412F, E11S/V42I/T57S/D118N/N168S/S188H/A195V/L311V, E11S/V42I/D53N/E68Y/N168S/S188H/A195V/L311V/Y412F, E11S/L19I/V42I/E68Y/D118N/S188H/A195V/L311V/Y412F, E11S/E68Y/S188H/A195V/L311V/Y412F, E11S/L19I/V42I/E68Y/L19I/D118N/N168S/S188H/A195V/L311V/Y412F, E11S/L19I/N168S/S188H/A195V/L311V/A317P/Y321F/Y412F, E11S/V42I/E68Y/Y412F, E11S/L19I/V42I/E68Y/Y412F, E11S/V42I/E68Y/S188H/A195V/Y412F, E11S/L19I/V42I/T57S/L19I/S188H/A195V/L311V/Y321W/Y412F, E11S/L19I/D53N/E68Y/S188H/A195V/L311V/Y412F, E11S/V42I/E68Y/S188H/A195V/L311V/Y321W/Y412F, E11S/V42I/E68Y/N168S/L311V/A317P/Y321W/Y412F, E11S/E68Y/L19I/S188H/A195V/L311V/A317P/Y321F/Y412F, E11S/V42I/T57S/E68Y/L19I/S188H/A195V/L311V/A317P/Y321F, E11S/L19I/L19I/S188H/A195V/L311V/A317P, E11S/V42I/E68Y/S188H/A195V/L311V/Y412F, E11S/V42I/L19I/S188H/A195V/L311V/A317P/Y321F/Y412F, E11S/Y412F, E11S/L19I/V42I/E68Y/L19I/S188H/A195V/Y412F, E11S/L19I/V42I/D53N/T57S/S188H/A195V/L311V/Y321W/Y412F, V42I/E68Y/Y412F, E11S/L19I/V42I/D53N/T57S/E68Y/L19I/N168S/S188H/A195V/L311V/A317P/Y321F, E11S/L19I/V42I/D53N/T57S/L19I/S188H/A195V/L311V/A317P/Y321W/Y412F, E11S/V42I/A317P/Y321F, E11S/V42I/T57S/E68Y/D118N/S188H/A195V/L311V/Y412F, E11S/V42I/D53N/E68Y/S188H/A195V/L311V/Y412F, E11S/L19I/D118N/S188H/A195V/L311V/Y412F, V42I/D53N/T57S/E68Y/M141L/N168S/T261A/Y321W/E381N/Y412F, E11S/V42I/S188H/L347N/Y412F/S434T, D118N/N168S/S188H/L347N/E348I/S382D/T388Q/V395L, E11S/E68Y/V88I/A317P/Y321F/S355D, E11S/L19I/E68Y/L19I/L79Y/M141L/N168S/T261A/A317P/Y321W/L347N, E11S/V42I/E68Y/L19I/L311V/Y321W/S434T, E11S/V42I/D53N/N168S/L311V/A317P/Y321W, E11S/E68Y/L19I/D118N/N168S/Y321F/Y412F, V42I/E68Y/L311V/L347N/L364I/Y412F, T57S/I69L/T89S/S188H/A195V/T261A/L311V/A317P/Y321W/F394Y/Y412F, Y321F/Y412F, E11S/V42I/E68Y/L311V/A317P/S434T, V42I/D53N/T57S/E68Y/I69L/S434T, V42I/E68Y/Y321W, L311V/L347N/E348I/S355D/L364I/S434T, V42I/E68Y/A317P/Y321F/Y412F, L19I/L19I/T89S/L311V/A317P/Y321F/S355D/L364I/Y412F, V42I/D53N/T57S/E68Y/L19I/M141L/S188H/A195V/L311V/A317P/Y321F/Y412F/S434T, E11S/L19I/V42I/D53N/E68Y/L311V/A317P/Y321W/Y412F, E11S/V42I/D53N/E68Y/L19I/S188H/A195V/E348I, E11S/T261A/L347N/E348I/S355D/S434T, E11S/A317P/Y321F/L347N, E11S/E68Y/L19I/T261A/L364I, E11S/L311V/Y321W/E348I/S355D/L364I/Y412F, V42I/D53N/T57S/L19I/V88I/L347N/E348I/F394Y/V395L/S434T, E11S/D53N/T89S/N168S/S188H/T261A/L311V/A317P/Y321W/S355D/Y412F, V42I/E68Y/T89S/M141L/T261A/L311V/Y321F/E348I/L364I, V42I/E68Y/D118N/S188H/T261A/Y412F/S434T, E11S/D53N/E68Y/L19I/T89S/Y321W/E348I/S355D, L311V/L347N/E348I, E11S/V42I/A317P/Y321F/L347N/Y412F, E11S/L19I/V42I/E68Y/A317P/Y321F/L364I/Y412F, D53N/E68Y/T89S/T261A, L19I/S188H/A195V/L311V/A317P/Y321W/V395L/Y412F, V42I/D53N/E68Y/V88I/T89S/D118N/L311V/Y412F/S434T, E11S/V42I/E68Y/L19I/L311V/E348I, V42I/Y321W, E68Y/T261A/L311V/L347N/E348I/S434T, E11S/E68Y/L19I/L79Y, and V42I/D53N/E68Y/S188H/A195V/T261A/L311V/Y321F/S355D/S434T.

A composition comprising a variant glucoamylase enzyme comprising at least one amino acid substitution as compared to SEQ ID NO:13, wherein said amino acid substitution is at a position selected from the group consisting of 5, 27, 36, 38, 46, 47, 50, 65, 68, 79, 89, 98, 108, 122, 124, 158, 161, 188, 192, 212, 215, 258, 262, 264, 298, 321, 400, 412, 420, 422, 457, 491, 497, 498, 503, 508, 509, 510, 516, 518, 521, 522, 526, 533, 534, 535, 540, 542, 544, 545, 549, 550, 551, 555, 556, 557, 559, 563, 564, 569, 570, 581 584, 7, 9, 11, 19, 26, 31, 42, 53, 55, 57, 69, 72, 73, 78, 85, 86, 88, 92, 93, 94, 97, 103, 106, 118, 125, 141, 145, 149, 150, 153, 155, 168, 170, 171, 178, 179, 186, 195, 204, 205, 208, 209, 213, 214, 219, 220, 224, 227, 246, 247, 256, 261, 306, 307, 311, 317, 342, 347, 348, 352, 353, 355, 362, 364, 370, 374, 381, 382, 388, 391, 394, 395, 399, 407, 418, 434, 436, 437, 440, 446, 452, 453, 460, 469, 470, 472, 475, 476, 478 and 492, wherein said variant glucoamylase enzyme has at least 1.1 fold better activity as compared to SEQ ID NO:13 under a condition selected from the group consisting of thermoactivity at about 30° C., thermoactivity at about 35° C., thermoactivity at about 40° C., thermoactivity at about 50° C., thermoactivity at about 60° C. and thermoactivity at about 70° C.;

• wherein said variant enzyme is at least 95% identical to SEQ ID NO:13, wherein the variant glucoamylase enzyme is not naturally occurring;
• and wherein amino acid substitutions are selected from the group consisting of T98S/Q161P/F516L, T98S/Q161P/N570P, T98S/Q161P, T550D/F555S, V65I/Y412F/Y503S/S518I, Y412F/Y503S/I564 T, Y412F/Y503E, Y412F/Y503E/S518I, V65I/Y412F/Y503S, V65I/Y503E, Y503D/S518I/I564N, Y503E/S518L, V65I/Y503E/S544Q V65I/Y503S, Y503E/S544E, V65I/Y503E/S544E/I564N, V65I/Y412F/Y503E, Y503E/S518I/S544Q, V65I/Y503E/S518M, Y412F/S518L, Y503E/S544Q, V65I/Y412F/Y503E/S544E/I564 T/G569I, Y412F/Y503S, Y503E/I564 T, S549D/F555D/G559N, S549D/F555R, K508T/L526N/S549D/G559N, K508T/G559N, F555D/I556P/G559N, S549D/F555R/I556P/G559N, F555R/I556P, L526I/F555R/I556P, F555R/I556P/G559N, K508T/I556P/G559N, F555D/I556P, K508T/L526N/A534T/S549D/I556P, K508T/L526I/S549D/G559N, K508N/L526I/S549E/F555L/G559N, L526N/S549D/F555D/I556P, L526I/F555D/G559N, A38V/F555R, L498T/T542N/T581N/T584N, T542N/T581N/T584N, S535Y/T542N, T540N/T584N, T540N/T542N/T581N/T584N, L498T/T581N/T584N, L498T/T584N, L498T/T542N/T581N, L498T/T542N, S535Y/T540N/T542N/T581N, Y321F/A422S, K5H/Y321W, K5N/Y321F, K5P/S188H, E68S/S188H/A422S, K5H/S188H/Y321F/A422S, S188H/A422S, S188H/A422S/V545A, K5Q/A422S, K5S/E68K/Y321W, K5H/Y321F/A422S, K5Y/A422S, K5Y/S188H/A422S, K5T/A422S, K5T/Y321W, Y321W/A422S, K5P/Y321F, K5H/S188H/Y321F/A422S, E68K/A422S, Y321F/A422S, K5H/Y321F, K5T/E68Y/S188T, K5S/E68Y/S188H, K5N/S188H, K5T/E68S/S188T, K5H/E68Y/S188P, K5P/E68Y/S188T, K5T/E68K/S188H, K5P/E68K/S188T, K5H/E68Y/S188T, K5T/S188H, K5T/E68N/S188T, K5Y/E68Y/S188T, K5S/E68Y/S188T, K5Q/E68K/S188H, K5P/E68N/S188H, K5P/E68N/S188T, K5P/E68Y/S188H, K5Y/E68Y/S188H, K5S/S188T, K5H/E68N/S188H, K5P/S188H, K5H/S46T/E68K/S188H, K5H/E68K/S188T, K5Q/E68Y/S188T, K5Y/E68N/S188H, L19I/V42I/E68Y/N168S/S188H/A195V/Y412F, E11S/V42I/T57S/D118N/N168S/S188H/A195V/L311V, E11S/V42I/D53N/E68Y/N168S/S188H/A195V/L311V/Y412F, E11S/L19I/V42I/E68Y/D118N/S188H/A195V/L311V/Y412F, E11S/E68Y/S188H/A195V/L311V/Y412F, E11S/L19I/V42I/E68Y/L19I/D118N/N168S/S188H/A195V/L311V/Y412F, E11S/L19I/N168S/S188H/A195V/L311V/A317P/Y321F/Y412F, E11S/V42I/E68Y/Y412F, E11S/L19I/V42I/E68Y/Y412F, E11S/V42I/E68Y/S188H/A195V/Y412F, E11S/L19I/V42I/T57S/169L/S188H/A195V/L311V/Y321W/Y412F, E11S/L19I/D53N/E68Y/S188H/A195V/L311V/Y412F, E11S/V42I/E68Y/S188H/A195V/L311V/Y321W/Y412F, E11S/V42I/E68Y/N168S/L311V/A317P/Y321W/Y412F, E11S/E68Y/169L/S188H/A195V/L311V/A317P/Y321F/Y412F, E11S/V42I/T57S/E68Y/L19I/S188H/A195V/L311V/A317P/Y321F, E11S/L19I/L19I/S188H/A195V/L311V/A317P, E11S/V42I/E68Y/S188H/A195V/L311V/Y412F, E11S/V42I/L19I/S188H/A195V/L311V/A317P/Y321F/Y412F, E11S/Y412F, E11S/L19I/V42I/E68Y/L19I/S188H/A195V/Y412F, E11S/L19I/V42I/D53N/T57S/S188H/A195V/L311V/Y321W/Y412F, V42I/E68Y/Y412F, E11S/L19I/V42I/D53N/T57S/E68Y/L19I/N168S/S188H/A195V/L311V/A317P/Y321F, E11S/L19I/V42I/D53N/T57S/L19I/S188H/A195V/L311V/A317P/Y321W/Y412F, E11S/V42I/A317P/Y321F, E11S/V42I/T57S/E68Y/D118N/S188H/A195V/L311V/Y412F, E11S/V42I/D53N/E68Y/S188H/A195V/L311V/Y412F, E11S/L19I/D118N/S188H/A195V/L311V/Y412F, V42I/D53N/T57S/E68Y/M141L/N168S/T261A/Y321W/E381N/Y412F, E11S/V42I/S188H/L347N/Y412F/S434T, D118N/N168S/S188H/L347N/E348I/S382D/T388Q/V395L, E11S/E68Y/V88I/A317P/Y321F/S355D, E11S/L19I/E68Y/L19I/L79Y/M141L/N168S/T261A/A317P/Y321W/L347N, E11S/V42I/E68Y/L19I/L311V/Y321W/S434T, E11S/V42I/D53N/N168S/L311V/A317P/Y321W, E11S/E68Y/L19I/D118N/N168S/Y321F/Y412F, V42I/E68Y/L311V/L347N/L364I/Y412F, T57S/L19I/T89S/S188H/A195V/T261A/L311V/A317P/Y321W/F394Y/Y412F, Y321F/Y412F, E11S/V42I/E68Y/L311V/A317P/S434T, V42I/D53N/T57S/E68Y/L19I/S434T, V42I/E68Y/Y321W, L311V/L347N/E348I/S355D/L364I/S434T, V42I/E68Y/A317P/Y321F/Y412F, L19I/L19I/T89S/L311V/A317P/Y321F/S355D/L364I/Y412F, V42I/D53N/T57S/E68Y/L19I/M141L/S188H/A195V/L311V/A317P/Y321F/Y412F/S434T, E11S/L19I/V42I/D53N/E68Y/L311V/A317P/Y321W/Y412F, E11S/V42I/D53N/E68Y/L19I/S188H/A195V/E348I, E11S/T261A/L347N/E348I/S355D/S434T, E11S/A317P/Y321F/L347N, E11S/E68Y/L19I/T261A/L364I, E11S/L311V/Y321W/E348I/S355D/L364I/Y412F, V42I/D53N/T57S/L19I/V88I/L347N/E348I/F394Y/V395L/S434T, E11S/D53N/T89S/N168S/S188H/T261A/L311V/A317P/Y321W/S355D/Y412F, V42I/E68Y/T89S/M141L/T261A/L311V/Y321F/E348I/L364I, V42I/E68Y/D118N/S188H/T261A/Y412F/S434T, E11S/D53N/E68Y/L19I/T89S/Y321W/E348I/S355D, L311V/L347N/E348I, E11S/V42I/A317P/Y321F/L347N/Y412F, E11S/L19I/V42I/E68Y/A317P/Y321F/L364I/Y412F, D53N/E68Y/T89S/T261A, L19I/S188H/A195V/L311V/A317P/Y321W/V395L/Y412F, V42I/D53N/E68Y/V88I/T89S/D118N/L311V/Y412F/S434T, E11S/V42I/E68Y/169L/L311V/E348I, V42I/Y321W, E68Y/T261A/L311V/L347N/E348I/S434T, E11S/E68Y/L19I/L79Y, and V42I/D53N/E68Y/S188H/A195V/T261A/L311V/Y321F/S355D/S434T.