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US 11,535,830 B2Granted
Threonine-producing yeast
AssigneeAdisseo France S.A.S.
InventorsDominique Louis, Karine Jaillardon, Dominique Thomas
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Abstract
A method for the bio-production of threonine including genetically modified yeasts and a method in which they are used to produce threonine, as compared to the parent yeasts.
Claims22
A threonine-producing recombinant yeast, in the genome of which:
- (A) at least one nucleic acid encoding an aspartate semi-aldehyde dehydrogenase that can use as coenzyme both NAD and NADP is overexpressed and/or is under the control of an inducible or repressible promoter;
- (B) at least one nucleic acid encoding an homoserine kinase is overexpressed and/or is under the control of an inducible or repressible promoter,
- (C) at least one nucleic acid encoding a threonine synthase is overexpressed and/or is under the control of an inducible or repressible promoter;
- (D) at least one nucleic acid encoding an aspartate kinase is overexpressed and/or is under the control of an inducible or repressible promoter;
- and
- (E) at least one nucleic acid encoding a glutamate dehydrogenase that converts oxo-glutarate to glutamate is overexpressed and/or is under the control of an inducible or repressible promoter,
- wherein:
- the yeast is of the genus Saccharomyces or Kluyveromyces, and
- the at least one nucleic acid encoding the aspartate semi-aldehyde dehydrogenase that can use as coenzyme both NAD and NADP is a nucleic acid sequence having at least 27% sequence identity with the nucleic acid sequence of SEO ID NO:1.
6 more elements
Dependencies are inferred from the claim text.