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US 11,535,828 B2Granted
Three-dimensional tissue
AssigneeRicoh Company, Ltd.
InventorsWaka Lin, Aino Hasegawa, Natsuko Hemmi, Satoshi Izumi, Shusaku Shiomoto
Life of this patent
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Abstract
Provided is a three-dimensional tissue, including: a first cellular region including cells of a first type; and a second cellular region including cells of a second type different from the first type, wherein the cells of the first type are cells that emit light by chemiluminescence, bioluminescence, or fluorescence in response to an external stimulus.
Claims13
A three-dimensional tissue, comprising:
- a first cellular region that comprises cells of a first type;
- and
- a second cellular region that comprises cells of a second type different from the first type,
- wherein the cells of the first type comprise a reporter gene linked downstream of a promoter of a target gene that emits light in response to an external stimulus, wherein the light is emitted by chemiluminescence, bioluminescence, or fluorescence,
- wherein the cells of the first type are absent in the second cellular region, and
- wherein the three-dimensional tissue is formed by
- dispensing a solution comprising collagen and the cells of the second type into a container and allowing the solution comprising collagen and the cells of the second type to solidify:
- dispensing a solution comprising fibrinogen on the solidified solution comprising cells of the second type followed by dispensing a mixture of the cells of the first type and thrombin;
- and
- subsequently dispensing an additional volume of the solution comprising collagen and the cells of the second type into the container, wherein the solution comprising collagen and the cells of the second type is allowed to solidify, and wherein the cells of the first type do not contact the container.
7 more elements
Dependencies are inferred from the claim text.