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US 11,535,663 B2Granted
Methods for producing aflibercept in chemically defined media having reduced aflibercept variants
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsShawn Lawrence, Amy Johnson, Meghan Casey, Jaimie Mastrogiacomo, Shunhai Wang, Ning Li
Life of this patent
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Abstract
The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.
Claims10
A method of producing aflibercept harvested from a host cell cultured in a chemically defined medium (CDM), comprising:
- a) culturing said host cell in said CDM under conditions suitable in which said host cell expresses said aflibercept, wherein the cumulative concentration of copper in said CDM is less than or equal to 0.8 μM or about 0.8 μM and the cumulative concentration of nickel in said CDM is less than or equal to 0.4 μM, and one or more of the following:
- i) the cumulative concentration of iron in said CDM is less than or equal to 55.0 μM;
- ii) the cumulative concentration of zinc in said CDM is less than or equal to 56.0 μM;
- iii) the cumulative concentration of cysteine in said CDM is less than or equal to 10.0 mM;
- and
- iv) said CDM includes anti-oxidants where the cumulative concentration of an antioxidant is about 0.001 mM to about 10.0 mM for any single anti-oxidant;
- and
- b) harvesting aflibercept produced by said host cell.
5 more elements
Dependencies are inferred from the claim text.