Cleavable peptides and insecticidal and nematicidal proteins comprising same
AssigneeVestaron Corporation
InventorsAlvar R. Carlson, Alexandra M. Haase, Robert M. Kennedy
Life of this patent
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Abstract
A peptide comprised of either a binary or a tertiary peptide, the peptide contains at least 4 amino acids and up to a maximum of 16 amino acids, comprised of 2 or 3 different regions, wherein the binary peptides have 2 different regions and the tertiary peptides have 3 different regions; wherein, the peptide can be cleaved by both an animal gut protease and an insect or nematode gut protease.
Claims21
A cleavable insecticidal or nematicidal polypeptide (CINP) comprising one or more cleavable peptide linkers (Linkers) said CINP having an Endoplasmic Reticulum Signal Peptide (ERSP) operably linked to the N-terminus of a construct comprising:
- (Linker-TP)n;
- (Linker-TP)n-Linker;
- (TP-Linker)n;
- or Linker-(TP-Linker)n;
- wherein the TP is a toxic protein, and n is an integer ranging from 2 to 200;
- and wherein the one or more Linkers has an amino acid sequence selected from the group consisting of:
- SEQ ID NOs:
- 1603, 1605, 1619, 1621, 1628, 1658, 1660, 1674, 1676, and 1683.
A plant, plant tissue, plant cell, plant seed, or part thereof comprising a polynucleotide encoding a CINP comprising one or more Linkers, wherein an ERSP is operably linked to the N-terminus of a construct comprising:
- (Linker-TP)n;
- (Linker-TP)n-Linker;
- (TP-Linker)n;
- or Linker-(TP-Linker)n;
- wherein TP is a toxic protein, and n is an integer ranging from 2 to 200;
- and wherein the one or more Linkers has an amino acid sequence selected from the group consisting of:
- SEQ ID NOs:
- 1603, 1605, 1619, 1621, 1628, 1658, 1660, 1674, 1676, and 1683.
A method of controlling a pest infection of a plant, said method comprising providing (1) a plant, plant tissue, plant cell, plant seed, or part thereof expressing a CINP;
- (2) a composition comprising a CINP which is applied to at least a portion of a plant, plant tissue, plant cell, plant seed, or plant part thereof to be consumed by the pest;
- or (3) combinations thereof;
- and wherein the CINP comprises one or more Linkers, wherein an ERSP is operably linked to the N-terminus of a construct comprising:
- (Linker-TP)n;
- (Linker-TP)n-Linker;
- (TP-Linker)n;
- or Linker-(TP-Linker)n;
- wherein TP is a toxic protein, and n is an integer ranging from 2 to 200;
- and wherein the one or more Linkers has an amino acid sequence selected from the group consisting of:
- SEQ ID NOs:
- 1603, 1605, 1619, 1621, 1628, 1658, 1660, 1674, 1676, and 1683.
Dependencies are inferred from the claim text.