Isolated darobactin A analog compounds and compositions thereof

A compound of Formula (I):

• and/or a pharmaceutically acceptable salt, stereoisomer, tautomer, or hydrate thereof, wherein:
• R1, R2, R3, R4, R5, and R6 are each independently selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, hydroxyl, hydroxyalkyl, halogen, —CN, —O-alkyl, —C(O)-alkyl, —C(O)O-alkyl, —C(O)OH, —C(O)NH2, —C(O)NH-alkyl, —NH2, —NO2, —CF3, —NH-alkyl, —N-(alkyl)2, —NHC(O)-alkyl and aryl, wherein said alkyl, alkenyl, alkynyl and aryl are each optionally substituted;
• R7 and R8 are each independently selected from the group consisting of hydrogen, straight or branched C1-C5 alkyl, straight or branched C2-C6 alkenyl, straight or branched C2-C6 alkynyl;
• wherein said alkyl, alkenyl and alkynyl are each optionally substituted;
• R9, R10, R11, R12, R13, R14, R15, R16, R17, R18, R19 and R20 are each independently selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, heterocycloalkyl, heterocycloalkenyl, aryl, heteroaryl, haloalkyl, hydroxyl, hydroxyalkyl, halogen, —CN, —O-alkyl, —C(O)-alkyl, —C(O)O-alkyl, —C(O)OH, —C(O)N H2, —C(O)NH-alkyl, —NH2, —NO2, —CF3, —NH-alkyl, —NHC(O)-alkyl, wherein said alkyl, alkenyl, alkynyl, cycloalkyl cycloalkenyl, heterocycloalkyl, heterocycloalkenyl, aryl, heteroaryl, haloalkyl are each optionally substituted;
• A is a bond or —(CH2)n—, wherein n is an integer between 0 and 10;
• and X is —C(O)—, —CH2—, —C(OH)—, —C(O)O-alkyl, —C((O)alkyl)-, with the provision that the compound of formula (I) is isolated in a substantially pure form from a microorganism;
• is encoded by a darA gene comprising at least one non-natural nucleotide substitution;
• and is neither naturally occurring nor the compound of formula (II):