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US 11,535,646 B2Granted
Method for purification of immunoglobulin
AssigneeThe Green Cross Corporation
InventorsSoo-kwang Kim, Kyung Hyun Boo, Kang Yun Seo, Sung Min Choi, Jeong Hye Yoon, Jin Hyun Park, Yeon Hui Kim
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Abstract
The present invention relates to an improved method of purifying an immunoglobulin, and more particularly to a method of purifying an immunoglobulin which is capable of sufficiently removing impurities from an immunoglobulin-containing plasma protein sample through a simple process, comprising a single anion-exchange chromatography and a single cation-exchange chromatography.
Claims8
A method of purifying an immunoglobulin, the method comprising:
- (a) subjecting an immunoglobulin-containing plasma sample to anion-exchange chromatography to obtain a fraction that is not attached to an anion-exchange chromatography column;
- and
- (b) subjecting the obtained fraction to cation-exchange chromatography,
- wherein the cation-exchange chromatography of process (b) above comprises eluting the immunoglobulin at a salt concentration of 200 mM to 300 mM, and
- wherein, after process (b) above, additional ion-exchange chromatography is not performed.
2 more elements
Dependencies are inferred from the claim text.