Method for preparing hexahydrofuro-furanol derivative, intermediate thereof and preparation method thereof

A method of preparation of an intermediate compound for the synthesis of (3R,3aS,6aR)-hexahydrofuro[2,3-b]furan-3-ol, comprising:

• enzymatically reducing a compound of formula (B) or a compound formula (B-2) to produce a compound of formula (C) or (C-2) respectively;
• wherein the enzyme is an aldehyde/ketone reductase having the amino acid sequence as shown in SEQ ID NO:1, or produced from the nucleotide sequence of the aldehyde/ketone reductase gene of SEQ ID NO:
• 2, and,
• wherein R1 is a hydrogen or a linear or branched acyl group of C2-11, a benzoyl group or a mono-substituted or multi-substituted benzoyl group on the benzene ring, and the mono-substituted or multi-substituted group is alkyl group, alkoxy group, nitro group or cyano group.