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US 11,534,753 B2Granted
Microfluidic kidney-on-chip
AssigneeEmulate, Inc.
InventorsVille Kujala, Hyoungshin Park, Sonalee Barthakur, Sauveur Jeanty, Brian Zuckerman, Josiah Sliz, Tanvi Shroff, Geraldine A Hamilton, Kyung-Jin Jang, Ananth Nookala, Gang Luo, Donald Mckenzie
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Abstract
The present invention relates to microfluidic fluidic devices, methods and systems as microfluidic kidney on-chips, e.g. human Proximal Tubule-Chip.
Claims17
A method of culturing, comprising:
- a) providing a microfluidic device comprising a membrane, said membrane comprising a first surface and a second surface, said first surface comprising proximal tubule cells and said second surface comprising glomerular microvascular endothelial cells;
- b) exposing said proximal tubule cells and said glomerular microvascular endothelial cells to media under continuous low shear flow;
- and
- c) culturing said proximal tubule cells such that said proximal tubule cells express aqua porin 1 (AQP1) with at least two-fold greater relative gene expression as compared to the same proximal tubule cells cultured in a static culture.
1 more element
A method of culturing, comprising:
- a) providing a microfluidic device comprising a membrane, said membrane comprising a first surface and a second surface, said first surface comprising human primary proximal tubular epithelial cells and said second surface comprising glomerular microvascular endothelial cells;
- b) culturing said human primary proximal tubular epithelial cells and said glomerular microvascular endothelial cells to media under continuous low shear flow such that said human primary proximal tubular epithelial cells express beta-catenin;
- and
- c) detecting said human primary proximal tubular epithelial cells expressing beta-catenin.
1 more element
A method of culturing, comprising:
- a) providing a microfluidic device comprising a membrane, said membrane comprising a first surface and a second surface, said first surface comprising human primary proximal tubular epithelial cells and said second surface comprising glomerular microvascular endothelial cells;
- b) culturing said human primary proximal tubular epithelial cells and said glomerular microvascular endothelial cells to media under continuous low shear flow such that said human primary proximal tubular epithelial cells express occludin;
- and
- c) detecting said human primary proximal tubular epithelial cells expressing occludin.
1 more element
Dependencies are inferred from the claim text.