Human nipple areolar complex extracellular matrix scaffold and methods relating thereto
AssigneeNovothelium, LLC
InventorsBianca G. Cerqueira, Lauren E. Cornell
Life of this patent
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Abstract
Methods of producing and methods of treatment using a nipple areolar complex extracellular matrix. A method for processing tissue comprises harvesting a human nipple areolar complex tissue, treating the tissue with a stabilizing medium, wherein the medium stabilizes the tissue during transport, decellularizing the tissue, and sterilizing the tissue to form a human nipple areolar complex extracellular matrix scaffold (ECMS). A method of treating an individual with a need for a nipple reconstruction comprises forming an ECMS and applying the ECMS to the individual for nipple areolar regeneration.
Claims23
A method for regenerating a nipple areolar complex comprising:
- a. harvesting a human nipple areolar complex tissue, wherein the human nipple areolar complex tissue comprises (i) an epidermal layer and (ii) dermal and subdermal layers;
- b. treating the tissue with a stabilizing medium, wherein the stabilizing medium stabilizes the tissue during a transport;
- c. decellularizing the tissue, wherein
- i. cells and DNA are removed, and
- ii. the epidermal layer is removed;
- and
- d. sterilizing the tissue;
- wherein said method yields a human nipple areolar complex extracellular matrix scaffold (ECMS) comprised of at least some of the dermal layers or subdermal layers without the epidermal layer, and
- wherein the human nipple areolar complex ECMS has reduced immunogenicity in humans as compared to native tissue.
A method of treatment comprising:
- a. identifying an individual with a need for a nipple reconstruction;
- b. forming a human nipple areolar complex extracellular matrix scaffold (ECMS), the forming comprising:
- i. harvesting a human nipple areolar complex tissue, wherein the human nipple areolar complex tissue comprises (i) an epidermal layer (ii) dermal and subdermal layers;
- ii. treating the tissue with a stabilizing medium, wherein the medium stabilizes the tissue during transport;
- iii. decellularizing the tissue, wherein
- A. cells and DNA are removed, and
- B. the epidermal layer is removed;
- and
- iv. sterilizing the tissue;
- wherein said forming of the human nipple areolar complex ECMS yields a sterile human nipple areolar complex ECMS comprising at least some of the dermal or subdermal layers without the epidermal layer;
- and
- c. applying the human nipple areolar complex ECMS to the individual.
A nipple areolar complex extracellular matrix scaffold (ECMS), comprising a decellularized human nipple areolar complex ECMS, wherein
- a. the nipple areolar complex ECMS comprises decellularized dermal or subdermal layers without an epidermal layer, and
- b. the nipple areolar complex ECMS is capable of supporting regeneration of a nipple areolar complex when implanted on a patient without generating a negative immune response in said patient.
Dependencies are inferred from the claim text.