Compositions and methods for stabilizing coelenterazine and analogs and derivatives thereof
AssigneePromega Corporation
InventorsMelanie Dart, Thomas Smith, Thomas Kirkland, Thomas Machleidt, Keith Wood
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Abstract
Provided herein are compositions and methods for stabilizing coelenterazine and analogs or derivatives thereof, and for improving the solubility and reconstitution efficiency of coelenterazine and analogs and derivatives thereof.
Claims17
A composition comprising:
- a compound selected from coelenterazine-h, coelenterazine-h-h, furimazine, JRW-0238, JRW-1743, and JRW-1744;
- and
- pullulan;
- wherein the composition is in the form of a lyophilized powder or cake.
A method of stabilizing a compound selected from coelenterazine-h, coelenterazine-h-h, furimazine, JRW-0238, JRW-1743, and JRW-1744, comprising:
- contacting the compound with an effective amount of pullulan;
- wherein the contacting step comprises:
- dissolving the compound in an organic solvent to form a first solution;
- mixing the first solution with the pullulan to form a mixture;
- and
- lyophilizing the mixture;
- to form a composition in the form of a lyophilized powder or cake.
A method of improving the solubility of a compound selected from coelenterazine-h, coelenterazine-h-h, furimazine, JRW-0238, JRW-1743, and JRW-1744, comprising:
- contacting the compound with an effective amount of pullulan;
- wherein the contacting step comprises:
- dissolving the compound in an organic solvent to form a first solution;
- mixing the first solution with the pullulan to form a mixture;
- and
- lyophilizing the mixture;
- to form a composition in the form of a lyophilized powder or cake.
A method of improving the reconstitution rate of a compound selected from coelenterazine-h, coelenterazine-h-h, furimazine, JRW-0238, JRW-1743, and JRW-1744, comprising:
- contacting the compound with an effective amount of pullulan;
- wherein the contacting step comprises:
- dissolving the compound in an organic solvent to form a first solution;
- mixing the first solution with the pullulan to form a mixture;
- and
- lyophilizing the mixture;
- to form a composition in the form of a lyophilized powder or cake,
- wherein the reconstitution rate for the compound is improved compared to a compound that has not been contacted with the pullulan.
Dependencies are inferred from the claim text.