Exosomes comprising therapeutic polypeptides
AssigneesEvox Therapeutics Ltd, Oxford University Innovation Limited
InventorsJustin Hean, Imre Mager, Matthew Wood, Samir El Andaloussi, Oscar Wiklander, Joel Nordin
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Abstract
The present invention pertains to an inventive release mechanism for extracellular vesicle (EV)-mediated intracellular and intramembrane delivery of therapeutic polypeptides. More specifically, the invention relates to EVs comprising polypeptide constructs which comprise a therapeutic polypeptide releasably attached to an exosomal polypeptide. Furthermore, the present invention pertains to manufacturing methods, pharmaceutical compositions, medical uses and applications, and various other embodiments related to the inventive EVs.
Claims12
An extracellular vesicle (EV) comprising at least one polypeptide construct comprising:
- (i) at least one polypeptide of interest (PoI);
- (ii) at least one exosomal polypeptide;
- and,
- (iii) a monomeric cis-cleaving intein,
- wherein the monomeric cis-cleaving intein releases the at least one PoI from the at least one exosomal polypeptide into the lumen of the EV upon activation within the EV, and
- wherein the at least one PoI is unconjugated upon release.
Dependencies are inferred from the claim text.