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US 11,534,496 B2Granted
Compositions and methods relating to T peripheral helper cells in autoantibody-associated conditions
AssigneeThe Brigham and Women's Hospital, Inc.
InventorsMichael B. Brenner, Deepak A. Rao
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Abstract
This disclosure provides methods and compositions for detecting Tph cells and/or reducing the number (or frequency) and/or activity of such cells in order to provide therapeutic benefit to a subject having or at risk of developing an autoantibody-associated condition such as an autoantibody-associated autoimmune disease.
Claims18
A method of detecting T peripheral helper (Tph) cells in a subject comprising:
- (i) obtaining a sample of cells from the subject;
- (ii) contacting the sample with:
- (a) an antibody specific for PD-1;
- (b) an antibody specific for CXCR5;
- (c) one or more antibodies each specific for a marker selected from the group consisting of:
- CCR2, CCR5 and CX3CR1;
- and
- (d) one or more antibodies each specific for a marker selected from the group consisting of:
- MHC Class II and ICOS;
- and
- (iii) immunodetecting Tph cells in the sample, wherein a Tph cell is characterized as having high expression of PD-1, low or no expression of CXCR5, expression of one or more of CCR2, CCR5 and CX3CR1, and expression of MHC Class II and/or ICOS.
8 more elements
Dependencies are inferred from the claim text.