Compositions and methods for modulating IL-10 immunostimulatory and anti-inflammatory properties

A method of increasing the anti-inflammatory window size of an IL-10 therapy in a patient suffering from an IL-10-related disease or disorder, comprising administering an effective amount to the patient a single chain IL-10 (scIL-10) polypeptide comprising an amino acid sequence arrangement from N-terminus to C-terminus in accordance with Formula 1:

• (first monomer subunit)-LINKER-(second monomer subunit) Formula 1
• wherein the first monomer subunit and the second monomer subunit may be independently selected from:
• SEQ ID NO:
• 1;
• or SEQ ID NO:
• 1 comprising at least one amino acid substitution, wherein the at least one amino acid substitution is at an amino acid position of SEQ ID NO:
• 1 that is selected from:
• amino acid position 22, amino acid position 41, amino acid position 87, and any combination thereof with the proviso that at least one of the first monomer subunit or the second monomer subunit comprises at least one amino acid substitution;
• wherein LINKER is an amino acid linker of between about 1 and about 100 amino acids in length;
• and
• wherein scIL-10 is optionally covalently attached to a fusion partner,
• thereby increasing the anti-inflammatory window size in the patient suffering from an IL-10-related disease or disorder.