Method for the stimulation of human immune cells

A method for producing a substance, the method comprising:

• leaching homogenate of Laminaria Japonica and Laminaria Angustata algae with a mixture of an aqueous solution and an isobutanolic solution, with a 1 g:2.5 ml:2.5 ml mix ratio, to obtain a homogeneous mixture;
• heating the homogenous mixture at 60° C. for one hour;
• incubating the homogenous mixture for 10-15 hours at room temperature to obtain a supernatant;
• withdrawing the supernatant from the homogenous mixture and centrifuging the supernatant at 3000 rpm and +4° C. for 30 minutes to obtain a centrifuged supernatant;
• purging the centrifuged supernatant with argon to obtain a purged, centrifuged supernatant and vacuum drying the purged, centrifuged supernatant for one hour at room temperature, followed by vacuum drying at 60° C. until a liquid fraction of the supernatant evaporates, to obtain a sediment;
• diluting the sediment with water to produce a 5% solution and then ultracentrifuging the 5% solution at 100,000 rpm for one hour to obtain a second supernatant;
• withdrawing the second supernatant and centrifuging the second supernatant in 10 kD filter tubes for one hour at 5000 rpm to obtain a lower percolated fraction;
• withdrawing the lower percolated fraction and centrifuging the lower percolated fraction in 5 kD filter tubes for one hour at 5000 rpm to obtain an upper fraction detained by a filter in the filter tubes;
• and
• withdrawing the upper fraction from the filter and diluting the upper fraction in 500 ml of NaCl saline to obtain a substance with a final concentration of 10 mg/ml.