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US 11,534,460 B2Granted
Method for expansion of double negative regulatory T cells
AssigneeUniversity Health Network
InventorsLi Zhang, Paulina Achita, Jong Lee, Dalam Ly, Dzana Dervovic
Life of this patent
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Abstract
There is provided herein a method for expanding human CD4-CD8- regulatory T cells (DN Tregs) from a population of cells comprising DN Tregs, comprising: culturing the population of cells with artificial antigen presenting cells (APCs), preferably the DN Tregs are αβ-TCR-CD56- or alternatively γ8-TCR+.
Claims21
A method for expanding human CD4− CD8− regulatory T cells (DN Tregs) from a population of cells comprising DN Tregs, comprising:
- depleting the population of CD4+ and CD8+ cells;
- activating the population with anti-CD3 antibodies;
- then
- culturing the population of cells with artificial antigen presenting cells (APCs) that express at least one cell surface adhesion molecule for immunological synapse, the at least one cell surface adhesion molecule comprising at least one of CD54 and CD58
- wherein the DN Tregs are αβ-TCR+CD56-, γδ-TCR+, or both.
2 more elements
Dependencies are inferred from the claim text.