Enhanced expression of RNA vectors
AssigneeThe University Court of the University of Edinburgh
InventorPeter Simmonds
Life of this patent
Estimating the term…
Abstract
The present invention relates to methods and compositions for enhancing expression from RNA expression vectores. The invention is based upon the observation that reducing the frequency of the dinucleotide CpG and UpA has a significant effect on expression from such vectores. Aspects of the invention include, amongst others, synthetic RNA vectores, virions, cells, methods of producing vaccines and methods of treatment or immunisation.
Claims20
A method of producing a synthetic RNA expression vector, the method comprising:
- modifying at least one region of a primary nucleotide sequence which reduces the frequency of at least one of CpG and UpA dinucleotides in said at least one region, thereby producing a modified primary nucleotide sequence;
- and
- producing a synthetic RNA expression vector comprising said modified primary nucleotide sequence which has a reduced frequency of at least one of CpG and UpA dinucleotides compared to a synthetic RNA expression vector which comprises an unmodified primary nucleotide sequence.
A method of producing a synthetic recombinant ssRNA virus genome, the method comprising:
- modifying at least one region of a primary ssRNA virus genome to reduce the frequency of CpG and UpA dinucleotides in said at least one region of the primary ssRNA virus genome, thereby producing one or more modified regions of the primary ssRNA virus genome;
- and
- producing a synthetic recombinant ssRNA virus genome comprising said one or more modified regions which have a reduced frequency of CpG and UpA dinucleotides compared to synthetic ssRNA virus genome which comprises an unmodified primary ssRNA virus genome.
Dependencies are inferred from the claim text.