Optical arrangement, multi-spot scanning microscope and method for operating a microscope

A multi-spot scanning microscope, comprising:

• a microscope optical unit,
• an illumination beam path,
• a detection beam path,
• a light source for providing excitation light in the illumination beam path,
• a scanner,
• an optical arrangement in the detection beam path, the optical arrangement including:
• a detection plane, in which a detector is positionable,
• a dispersive device for spectrally separating detection light,
• a distortion optical unit for guiding the detection light into the detection plane, said distortion optical unit being arranged, in the beam direction, downstream of the dispersive device and upstream of the detection plane, and
• a rotation device for the relative rotation of a luminous field of the spectrally separated detection light and the distortion optical unit,
• a detector positioned in the detection plane of the optical arrangement, wherein the detector plane of the optical arrangement is optically conjugate to a sample plane, and
• a control unit for actuating the optical arrangement and the light source and for at least provisionally processing measurement data provided by the detector.