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US 11,531,035 B2Granted
Calcium indicator polypeptides and methods of use thereof
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsKarl A. Deisseroth, Charu Ramakrishnan, Masatoshi Inoue
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Abstract
The present disclosure provides a genetically encoded calcium indicator (GECI), nucleic acids encoding the GECI, and host cells comprising the GECI. The present disclosure also provides methods of detecting a change in the intracellular concentration of a cell expressing a GECI of the present disclosure.
Claims18
A genetically encoded calcium indicator (GECI) as set forth in SEQ ID NO:
- 1, wherein there is
- an aspartic acid at an amino acid position corresponding to amino acid 217 of SEQ ID NO:1;
- or a variant thereof, wherein the variant comprises a calmodulin polypeptide comprising one or more of:
- a) an Asp→Gln substitution at position D272;
- b) a Phe→Met substitution at an amino acid corresponding to F305 of the amino acid sequence set forth in SEQ ID NO:
- 1;
- c) a Phe→Leu substitution at an amino acid corresponding to F305 of the amino acid sequence set forth in SEQ ID NO:
- 1;
- d) an Ile→Met substitution at an amino acid corresponding to I296 of the amino acid sequence set forth in SEQ ID NO:
- 1;
- and
- e) a Leu→Ile substitution at an amino acid corresponding to L385 of the amino acid sequence set forth in SEQ ID NO:
- 1.
10 more elements
Dependencies are inferred from the claim text.