Back to results
US 11,531,034 B2Granted
Compositions and methods for lysis of red blood cells
AssigneeBeckman Coulter, Inc.
InventorsGeorge C. Brittain, IV, Sergei Gulnik
Life of this patent
Estimating the term…
Abstract
This disclosure provides methods and kits for lysing red blood cells from whole blood.
Claims12
A method of lysing red blood cells comprising:
- contacting a sample comprising red blood cells and white blood cells with a first buffer comprising about 0.5% to 10% formaldehyde, 0.01 mM to 20 mM ethylenediaminetetraacetic acid (EDTA) and 150 mM to 600 mM salt at a pH of 6.5 to 8.5,
- wherein the solute concentration of the first buffer is greater than the solute concentration of the sample, and wherein the sample and the first buffer form a first mixture;
- adding a second buffer comprising 0.5× to 1× phosphate-buffered saline (PBS) to the first mixture to form a second mixture, wherein the solute concentration in the second mixture is at least 90% of the solute concentration of the sample and wherein the red blood cells in the sample are lysed.
A method of processing a whole blood sample comprising:
- forming a first mixture comprising a sample of whole blood, about 0.5% to 10% formaldehyde, a hypertonic saline having a salt concentration of 150 mM to 600 mM and is at pH of 6.5 to 8.5, and 0.01 mM to 20 mM ethylenediaminetetraacetic acid (EDTA),
- wherein the solute concentration of the hypertonic saline is greater than the solute concentration of the whole blood;
- and
- adding a second buffer comprising 0.5× to 1× phosphate-buffered saline (PBS) to the first mixture to form an isotonic second mixture.
1 more element
A red cell lysis kit comprising:
- a first reagent comprising a hypertonic saline, 0.5% to 10% formaldehyde, and 0.01 mM to 20 mM ethylenediaminetetraacetic acid (EDTA),
- wherein the solute concentration of the first reagent is greater than the solute concentration of a whole blood sample;
- and
- a second reagent including 0.5× to 1× phosphate-buffered saline (PBS),
- wherein the hypertonic saline has a salt concentration of 150 mM to 600 mM and is at pH of 6.5 to 8.5.
2 more elements
Dependencies are inferred from the claim text.