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US 11,531,032 B2Granted
Methods for measuring analyte and/or protein in biological samples
AssigneeIDEXX Laboratories, Inc.
InventorsPatrick Doherty, Kevin Kirspel, Giosi Farace, Murthy VSN Yerramilli
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Abstract
The disclosure directed to methods for measuring an analyte alone or in combination with total protein in biological samples. More particularly, the disclosure relates to methods for measuring an analyte and/or total protein using one or more colorimetric reagents alone or in combination with protein precipitation reagents.
Claims30
A method of measuring a concentration of an analyte or total protein in a biological sample, the method comprising:
- contacting the biological sample with a colorimetric reagent to obtain a processed sample;
- producing an image of the processed sample having a predetermined level of exposure by focusing a camera comprising an aperture at the processed sample;
- and opening the aperture for a period of time sufficient to collect a predetermined amount of light from the processed sample to produce the image;
- measuring the period of time that the aperture is open to collect the predetermined amount of light;
- and
- correlating the period of time with the concentration of the analyte or the total protein in the biological sample,
- wherein the predetermined amount of light is collected regardless of processed sample color.
4 more elements
A method of measuring a concentration of an analyte and total protein in a biological sample, the method comprising:
- contacting the biological sample with a colorimetric reagent and a protein precipitation reagent to obtain a processed sample comprising a protein precipitate;
- determining the concentration of the analyte in the processed sample by producing a first image of the processed sample having a predetermined level of exposure by focusing a camera comprising an aperture at the processed;
- sample and opening the aperture for a period of time sufficient to collect a predetermined amount of light from the processed sample to produce the image, wherein the predetermined amount of light is collected regardless of processed sample color;
- measuring the period of time that the aperture is open to collect the predetermined amount of light;
- and
- correlating the period of time with the concentration of the analyte in the biological sample;
- and
- determining the concentration of total protein by
- generating a second image of the processed sample with the camera;
- measuring a number of pixels that correlates to the protein precipitate in the image;
- and
- correlating the number of pixels with a standard curve to obtain the concentration of total protein in the biological sample.
9 more elements
Dependencies are inferred from the claim text.