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US 11,531,029 B2Granted
Methods and compositions for the detection of flavivirus infections
AssigneeUniversity of Pittsburgh—Of the Commonwealth System of Higher Education
InventorsErnesto Torres De Azeved Marques, Jr., Eduardo Nascimento, Albert Icksang Ko, Donald S. Burke
Life of this patent
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Abstract
Disclosed are compositions and methods for the detection of a Flavivirus infection. In some embodiments, the method comprises detecting a recent Flavivirus infection by measuring the amount of anti-NS1 IgG3. In other embodiments, the method comprises detecting a prior Dengue virus infection in a subject previously immunized with a Dengue virus vaccine comprising one or more non-Dengue Flavivirus proteins.
Claims20
A method of measuring efficacy of a recently administered Flavivirus vaccine comprising:
- a) obtaining a biological sample from a subject administered the Flavivirus vaccine within a prior four months and wherein the subject had an onset of symptoms of a Flavivirus a infection greater than four months prior to the administration of the Flavivirus vaccine;
- b) contacting the biological sample with one or more Flavivirus NS1 polypeptides corresponding to the Flavivirus vaccine;
- and
- c) detecting an amount of anti-NS1 IgG3 in the biological sample;
- wherein an increase in the amount of anti-NS1 IgG3 relative to a control indicates efficacy of the recently administered Flavivirus vaccine, wherein the control is obtained from a second subject or a population that had the Flavivirus infection and had not been recently vaccinated with the Flavivirus vaccine.
2 more elements
Dependencies are inferred from the claim text.