Formation and use of embedded solutions in nanoscale materials
AssigneeThe Regents of the University of California
InventorTing Guo
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Abstract
Provided herein are methods and materials that allow targeting and imaging of interactions between probes and targets. In some embodiments, the probes include nanoscale materials with embedded solutions that can be used to measure physical enhancement by materials under X-ray irradiation. In some embodiments, the methods of the present invention include delivering a probe material to a target that can have a delivered donor material. In some embodiments, methods of the present invention include irradiating the target and determining an optical change in the probe characteristic of a physical enhancement.
Claims20
A method of confirming a targeting operation, the method comprising:
- delivering a donor material to a target;
- delivering a probe to the target, wherein the probe couples with the donor material;
- irradiating the target;
- determining an optical change in the probe to confirm the donor material and probe reached the target;
- delivering a solution to dislodge the probe from the donor material;
- and
- extracting the probe from the target, wherein the target is in a living organism.
A method of determining conjugation between a probe nanomaterial and a donor nanomaterial, the method comprising:
- functionalizing a donor nanomaterial to target a substance, wherein the donor nanomaterial comprises at least one material selected from the group consisting of lanthanide oxide, hafnium oxide, tungsten oxide, platinum, gold, bismuth, and uranium-238, and wherein the donor nanomaterial comprises a nanomaterial having a diameter between about 25 nm and about 1,000 nm;
- delivering the donor nanomaterial to a location;
- functionalizing a probe nanomaterial to target the donor nanomaterial;
- delivering the probe nanomaterial to the location;
- irradiating the location with radiation from a source selected from the group consisting of an X-ray source, a microwave source, or a gamma ray source;
- testing the probe nanomaterial to identify a change in optical characteristics;
- determining successful conjugation between the probe nanomaterial and the donor nanomaterial at the location;
- and based on determining successful conjugation, determining the substance exists at the location.
A method of confirming a targeting operation, the method comprising:
- delivering a donor material to a target;
- delivering a probe to the target, wherein the probe comprises at least one material selected from the group consisting of TiO2, ZnO, Al2O3, SiO2, CaP, polystyrene, and poly N-isopropylacrylamide, wherein the probe further comprises at least one material selected from the group consisting of coumarin-3-carboxylic acid, 3′-(p aminophenyl) fluorescein, 2-[6-(4V-amino)phenoxy-3H-xanthen-3-on-9-yl] benzoic acid (APF), dihydroethidine (DHE), dihydrorhodamine, 4′,5′-diaminofluorescein, sulforhodamine B, calcein, and fluorescein, and wherein the probe is characterized by a diameter of between about 2 nm and about 500 nm;
- irradiating the target with radiation from a source selected from the group consisting of an X-ray source, a microwave source, or a gamma ray source;
- and
- determining an optical change in the probe to confirm the donor material and probe reached the target.
Dependencies are inferred from the claim text.