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US 11,531,005 B2Granted
High-throughput label-free enzymatic bioassays using DESI-MS
AssigneePurdue Research Foundation
InventorsRobert Graham Cooks, Nicolás M. Morato, Dylan T. Holden
Life of this patent
Estimating the term…
Abstract
The invention generally relates to high-throughput label-free enzymatic bioassays using desorption electrospray ionization-mass spectrometry (DESI-MS).
Claims19
A method for analyzing one or more enzymatic reactions, the method comprising:
- preparing a plurality of discrete spots on a substrate, wherein each of the discrete spots comprises a substrate and a product of one or more enzymatic reactions, wherein both of the substrate and the product of the one or more enzymatic reactions are label-free, and wherein a subset of each of the plurality of the discrete spots further comprises a same test compound;
- directing sequentially a discharge from an ionization source onto each of the plurality of discrete spots to sequentially desorb the substrate and/or product from each of the discrete spots and sequentially generate ions of the substrate and/or product from each of the discrete spots that enter a mass spectrometer;
- analyzing sequentially the ions of the substrate and/or product from each of the discrete spots in the mass spectrometer to thereby analyzing the one or more enzymatic reactions;
- and
- determining whether the test compound inhibits the enzymatic reaction based on monitoring of the progress of the enzymatic reaction.
2 more elements
A method for analyzing one or more enzymatic reactions, the method comprising:
- preparing a plurality of discrete spots on a substrate, wherein each of the discrete spots comprises a substrate and a product of one or more enzymatic reactions, wherein both of the substrate and the product of the one or more enzymatic reactions are label-free and wherein a subset of each of the plurality of the discrete spots further comprises an inhibitor of an enzyme of the enzymatic reaction and a test compound;
- directing sequentially a discharge from an ionization source onto each of the plurality of discrete spots to sequentially desorb the substrate and/or product from each of the discrete spots and sequentially generate ions of the substrate and/or product from each of the discrete spots that enter a mass spectrometer;
- analyzing sequentially the ions of the substrate and/or product from each of the discrete spots in the mass spectrometer to thereby analyzing the one or more enzymatic reactions;
- and
- determining whether the test compound can counteract the inhibitor and re-start the enzymatic reaction.
2 more elements
A method for analyzing one or more enzymatic reactions, the method comprising:
- preparing a plurality of discrete spots on a substrate, wherein each of the discrete spots comprises a substrate and a product of one or more enzymatic reactions, wherein both of the substrate and the product of the one or more enzymatic reactions are label-free;
- directing sequentially a discharge from an ionization source onto each of the plurality of discrete spots to sequentially desorb the substrate and/or product from each of the discrete spots and sequentially generate ions of the substrate and/or product from each of the discrete spots that enter a mass spectrometer;
- obtaining sequentially ion intensities of the ions of the substrate and/or product from each of the discrete spots in the mass spectrometer;
- and
- converting ion intensities of the ions of the substrate and/or product from each of the discrete spots into concentration ratios of the substrate and product by applying a calibration curve, thereby analyzing the one or more enzymatic reactions.
2 more elements
Dependencies are inferred from the claim text.