Fluorescence triple-correlation spectroscopy system for analyzing interaction between three kinds of molecules
AssigneeShanghai Jiao Tong University
InventorsChaoqing Dong, Jicun Ren
Life of this patent
Estimating the term…
Abstract
A ternary fluorescence correlation spectroscopy system for analyzing an interaction between three kinds of molecules, including at least three excitation light sources with different wavelengths. The excitation light sources are configured to illuminate and excite a sample to generate a fluorescence signal. The fluorescence signal is divided into multiple signals according to wavelength, which are then respectively detected by a single photon detector and transmitted to a signal acquisition and operation card to perform real-time operation of a triple-correlation function, so as to obtain a fluorescence triple-correlation spectroscopy curve.
Claims8
A fluorescence triple-correlation spectroscopy system for analyzing an interaction between three kinds of molecules, comprising:
- at least three excitation light sources varying in emission wavelength;
- wherein the at least three excitation light sources are configured to coaxially emit laser beams to illuminate and excite a sample to generate a fluorescence signal;
- the fluorescence signal is divided into a plurality of signal detection channels by a plurality of first dichroic mirrors according to wavelength and then respectively transmitted to a single photon detector for signal detection, and then transmitted to a signal acquisition and operation card;
- the signal acquisition and operation card is connected to the single photon detector, and the signal acquisition and operation card is configured for real-time operation, to obtain a fluorescence triple-correlation spectroscopy curve;
- the at least three excitation light sources each comprise a first laser, a second laser and a third laser, wherein the first laser is configured to emit a 488 nm laser beam, the second laser is configured to emit a 561 nm laser beam, and the third laser is configured to emit a 640 nm laser beam;
- the real-time operation is performed using a triple-correlation function expressed as follows:
- G
- 2
- ⋆
- 3
- ⋆
- 4
- (
- τ
- 1
- ,
- τ
- 2
- )
- =
- 〈
- δ
-
- I
- 2
- (
- t
- )
-
- δ
-
- I
- 3
- (
- t
- +
- τ
- 1
- )
-
- δ
-
- I
- 4
- (
- t
- +
- τ
- 2
- )
- 〉
- 〈
- I
- 2
- (
- t
- )
- 〉
-
- 〈
- I
- 3
- (
- t
- )
- 〉
-
- 〈
- I
- 4
- (
- t
- )
- 〉
- ;
- wherein t is time;
- I2(t) indicates a fluorescence intensity fluctuation signal excited by the 488 nm laser beam;
- I3(t) indicates a fluorescence intensity fluctuation signal excited by the 561 nm laser beam;
- and I4(t) indicates a fluorescence intensity fluctuation signal excited by the 640 nm laser beam
- wherein τ1 indicates a delay time when a 561 nm detection channel is correlated with a 488 nm detection channel;
- and τ2 indicates a delay time when a 640 nm detection channel is correlated with the 488 nm detection channel.
Dependencies are inferred from the claim text.