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US 11,530,449 B2Granted
Detecting neoplasm
AssigneeMayo Foundation for Medical Education and Research
InventorsWilliam R. Taylor, Jonathan J. Harrington, Patrick S. Quint, Hongzhi Zou, Harold R. Bergen, III, David I. Smith, David A. Ahlquist
Life of this patent
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Abstract
This document relates to methods and materials for detecting premalignant and malignant neoplasms. For example, methods and materials for determining whether or not a stool sample from a mammal contains nucleic acid markers or polypeptide markers of a neoplasm are provided.
Claims8
A system, comprising:
- (a) reagents for extracting genomic DNA from a biological sample of a human individual suspected of having precancer or colorectal cancer;
- (b) primers specific for K-ras, wherein the primers specific for K-ras are selected from SEQ ID NOs:
- 2, 3, 4, 5, 6, 7 and 8;
- (c) reagents sufficient for measuring the methylation level of one or more CpG sites in BMP3,
- wherein the reagents sufficient for measuring the methylation level of one or more CpG sites in BMP3 comprise reagents sufficient for conducting one or more of methylation-specific PCR, quantitative methylation-specific PCR, methylation-sensitive DNA restriction enzyme analysis, quantitative bisulfite pyrosequencing, and bisulfite genomic sequencing PCR,
- wherein the reagents sufficient for conducting one or more of methylation-specific PCR, quantitative methylation-specific PCR, methylation-sensitive DNA restriction enzyme analysis, quantitative bisulfite pyrosequencing, and bisulfite genomic sequencing PCR comprise BMP3 specific primers and a bisulfite reagent;
- and
- (d) a stool stability buffer.
5 more elements
Dependencies are inferred from the claim text.