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US 11,530,445 B2Granted
Methods and compositions for delivery of molecule and complexes to reaction sites
AssigneePacific Biosciences of California, Inc.
InventorStephen Turner
Life of this patent
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Abstract
The present invention provides methods, compositions, and systems for distributing molecules and complexes into reaction sites. In particular, the methods, compositions, and systems of the present invention result in an active loading of molecules and complexes into reaction sites with improved efficiency over loading by passive diffusion methods alone.
Claims13
A method of delivering template nucleic acids to nanoscale wells, the method comprising:
- (a) providing an array of nanoscale wells;
- (b) providing at least one channel over the array of nanoscale wells, wherein the at least one channel comprises regions of greater and lesser confinement;
- (c) applying a solution comprising template nucleic acids to the at least one channel;
- (d) applying a driving force to the solution to move the template nucleic acids through the channel over the nanoscale wells, wherein the regions of greater confinement serve as entropic barriers to delay or substantially prevent progress of the template nucleic acids along the channel;
- wherein the regions of lesser confinement are located over the nanoscale wells and the regions of greater confinement are located between the nanoscale wells, such that the entropic barriers posed by the regions of greater confinement serve to concentrate the template nucleic acids over the nanoscale wells in the at least one channel as compared to between the wells in the at least one channel;
- and
- (e) allowing the template nucleic acids to passively diffuse from the at least one channel into the nanoscale wells,
- thereby delivering template nucleic acids to nanoscale wells.
5 more elements
Dependencies are inferred from the claim text.