Cell mass evaluation method and device for analyzing state of cell mass
AssigneeHamamatsu Photonics K.K.
InventorsKentaro Goto, Toyohiko Yamauchi, Hidenao Yamada
Life of this patent
Estimating the term…
Abstract
An evaluation method is a method for evaluating a cell mass containing a plurality of aggregated cells, and includes an imaging step of capturing an image of light obtained from the cell mass by irradiating the cell mass with light, an analysis step of setting a reference point for the cell mass included in image data obtained by the imaging step, setting sampling circles centered on the reference point, and determining a parameter for a state of the cell mass based on image data included in regions on the sampling circles, and an evaluation step of evaluating the cell mass based on the parameter.
Claims21
A cell mass evaluation method for evaluating a cell mass containing cells aggregated with each other, the method comprising:
- an imaging step of capturing an image of light obtained from the cell mass by irradiation with light to the cell mass;
- an analysis step of setting a reference point for the cell mass included in the image obtained by the imaging step, setting a sampling circle centered on the reference point, and determining a parameter for a state of the cell mass based on image data included in a region on the sampling circle;
- and
- an evaluation step of evaluating the cell mass based on the parameter, wherein
- the parameter is a width of a first waveform of an autocorrelation function of the image data along a circumferential direction of the sampling circle.
A cell mass state analysis apparatus used for evaluating a cell mass containing cells aggregated with each other, the apparatus comprising:
- a light source configured to output light with which the cell mass is irradiated;
- an imager configured to capture an image of light obtained from the cell mass by irradiation with the light;
- and
- an analyzer configured to set a reference point for the cell mass included in the image obtained by the imaging, set a sampling circle centered on the reference point, and determine a parameter for a state of the cell mass based on image data included in a region on the sampling circle, wherein
- the parameter is a width of a first waveform of an autocorrelation function of the image data along a circumferential direction of the sampling circle.
A cell mass evaluation method for evaluating a cell mass containing cells aggregated with each other, the method comprising:
- an imaging step of capturing an image of light obtained from the cell mass by irradiation with light to the cell mass;
- an analysis step of setting a reference point for the cell mass included in the image obtained by the imaging step, setting a sampling circle centered on the reference point, and determining a parameter for a state of the cell mass based on image data included in a region on the sampling circle;
- and
- an evaluation step of evaluating the cell mass based on the parameter, wherein
- the parameter is a cell number density in the region on the sampling circle.
A cell mass state analysis apparatus used for evaluating a cell mass containing cells aggregated with each other, the apparatus comprising:
- a light source configured to output light with which the cell mass is irradiated;
- an imager configured to capture an image of light obtained from the cell mass by irradiation with the light;
- and
- an analyzer configured to set a reference point for the cell mass included in the image obtained by the imaging, set a sampling circle centered on the reference point, and determine a parameter for a state of the cell mass based on image data included in a region on the sampling circle, wherein
- the parameter is a cell number density in the region on the sampling circle.
Dependencies are inferred from the claim text.