Method for preparing isomaltooligosacharide composition

A method for producing an isomaltooligosaccharide composite comprising 10 to 35% (w/w) isomaltooligosaccharide and 0% (w/w) isomaltose of the total weight of the composite based on the solid weight of the composite, comprised of:

• a first step to produce a liquefied solution by contacting a starch slurry to a liquifying enzyme;
• a second step to produce an isomaltooligosaccharide saccharified solution by contacting a primary saccharogenic enzyme and a secondary saccharogenic enzyme with the liquefied solution;
• and followed by
• a third step to produce fructose of 30 to 40% (w/w) based on the total solid weight by contacting isomerase with the isomaltooligosaccharide saccharified solution;
• wherein the primary saccharogenic enzyme is a glucoamylase, alpha-amylase, pullulanase, or a combination thereof,
• wherein the secondary saccharogenic enzyme is a transglucosidase,
• wherein the secondary saccharogenic enzyme is in an amount from 0.03 to 0.08% (w/w) based on the total solid weight of the liquefied solution,
• wherein the primary and secondary saccharogenic enzymes are contacted with the liquified solution for 24 hours to 72 hours at pH 4.7 to 5.5, and at a temperature from 55 to 60° C., and
• wherein the isomaltooligosaccharide composite is comprised of from 85 to 97% (w/w) of monosaccharides or disaccharides.