Method for biohydrogen production

A method, comprising:

• 1. mixing a hydrogen production medium and a buffer solution Na2HPO4/NaH2PO4 having a pH value of between 5 and 9, to yield a first mixture;
• 2. adding corn stalk powder and cellulase to the first mixture and mixing, to yield a second mixture;
• 3. culturing photosynthesis bacteria in a culture media until a late exponential phase of growth, adding a suspension of the photosynthesis bacteria at the late exponential phase of growth to the second mixture, to yield a third mixture, wherein the photosynthesis bacteria comprise Rhodospirillum cristatum, Rhodopseudomonas capsulatus, Rhodopseudomonas palustris, Rhodobacter sphaeroides, and Rhodobacter capsularis;
• and
• 4. sealing the third mixture and allowing for photo-fermentation biohydrogen production under anaerobic fermentation conditions.