Pathogen resistance in plants
AssigneeFortiphyte, Inc.
InventorsAlexander Christiaan Schultink, Laura A. Wetzel
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Abstract
The present disclosure provides an isolated, recombinant, or synthetic polynucleotide comprising a FIT1 protein, and homologs, fragments, and variations thereof. The disclosure further relates to transgenic plants, plant parts, and plant cells comprising one or more of these polynucleotides, and exhibit resistance or tolerance to a pathogen, such as Phakopsora pachyrhizi. The disclosure further relates to methods of genetically engineering a pathogen resistance or tolerance trait in a plant, plant part, or plant cell, comprising targeted gene editing of a FIT1 homolog, and plants produced therefrom. The disclosure further relates to methods for identifying new functional FIT1 genes and/or alleles thereof.
Claims22
A recombinant DNA construct comprising a nucleic acid sequence, wherein the nucleic acid sequence encodes a protein comprising SEQ ID NO:
- 6, or a protein at least 90% identical thereto, and wherein the construct is capable of conferring resistance to a fungal pathogen when transformed into a plant.
A transgenic plant, plant part, or plant cell having resistance or tolerance to a fungal pathogen, wherein the resistance or tolerance is conferred by a transgene comprising a nucleic acid sequence encoding a protein comprising SEQ ID NO:
- 6, or a protein at least 90% identical thereto.
A method of producing a plant, plant part, or plant cell having resistance or tolerance to a fungal pathogen, wherein the method comprises:
- transforming a plant, plant part, or plant cell with an isolated, recombinant, or synthetic polynucleotide comprising:
- a nucleic acid sequence encoding a functional FIT1 protein, wherein the protein is at least 90% identical to SEQ ID NO:
- 6 and
- selecting a plant comprising the polynucleotide and having resistance or tolerance to a fungal pathogen.
Dependencies are inferred from the claim text.