Prunus persica polygalacturonase-inhibiting protein 1 (PpPGIP1) gene, and cloning method and use thereof

A method of conferring antifreeze in a peach, comprising:

• introducing a virus-induced Prunus persica polygalacturonase-inhibiting protein 1 (PpPGIP1) gene silencing vector in the peach;
• and
• reducing an expression level of a PpPGIP1 gene by the virus-induced PpPGIP1 gene silencing vector to reduce a Prunus persica vacuolar invertase 2 (PpVIN2) activity and decomposition of a sucrose in the peach,
• wherein the PpPGIP1 gene comprises the nucleotide sequence shown in of SEQ ID NO:
• 1,
• wherein the steps for introducing the virus-induced PpPGIP1 gene silencing vector in the peach comprise:
• designing and amplifying a silencing fragment sequence of the PpPGIP1 gene;
• subcloning the silencing fragment sequence into a first virus-induced gene silencing vector to obtain a recombinant vector;
• respectively transforming the recombinant vector and a second virus-induced gene silencing vector into Agrobacterium to obtain a first transformant and a second transformant;
• and
• infecting the first transformant and the second transformant into the peach to introduce the virus-induced PpPGIP1 gene silencing vector in the peach.