Enzyme immobilization in hierarchical metal-organic frameworks
AssigneeNorthwestern University
InventorsOmar K. Farha, Peng Li, Justin A. Modica, Milan Mrksich, Joseph T. Hupp
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Abstract
Enzyme-immobilizing MOFs and methods for their use in enzymatically catalyzed reactions are provided. The MOFs are channel-type MOFs that present a hierarchical pore structure comprising a first set of large channels sized for enzyme immobilization and a second set of smaller channels running alongside of the large channels that remain enzyme-free and allow for reactant delivery to the enzymes and product expulsion from the larger channels.
Claims18
An enzyme-immobilizing metal-organic framework compound of comprising:
- a channel-type metal-organic framework compound comprising Zr6 cluster nodes and having a hierarchical pore structure comprising a first set of large channels, a second set of small channels running alongside of the large channels, and openings defined between the large channels and the small channels, the large channels having a larger diameter than the small channels, wherein the channel-type metal-organic framework compound has a csq-net topology in which the large channels have a hexagonal cross-section and the small channels have a triangular cross-section;
- and
- catalytically active enzymes immobilized in the large channels.
An enzyme-immobilizing metal-organic framework compound of comprising:
- a channel-type metal-organic framework compound comprising Zr6 cluster nodes and having a hierarchical pore structure comprising a first set of large channels, a second set of small channels running alongside of the large channels, and openings defined between the large channels and the small channels, the large channels having a larger diameter than the small channels, wherein the channel-type metal-organic framework compound has a length in the range from 100 nm to 1000 nm;
- and
- catalytically active enzymes immobilized in the large channels.
A method of enzymatically catalyzing a reaction using enzyme-immobilizing metal-organic framework compounds comprising:
- channel-type metal-organic framework compound comprising Zr6 cluster nodes and having a hierarchical pore structure comprising a first set of large channels, a second set of small channels running alongside of the large channels, and openings defined between the large channels and the small channels, the large channels having a larger diameter than the small channels, wherein the channel-type metal-organic framework compound has a csq-net topology in which the large channels have a hexagonal cross-section and the small channels have a triangular cross-section;
- and
- catalytically active enzymes immobilized in the large channels of the channel-type metal-organic framework compounds,
- the method comprising:
- exposing the enzyme-immobilizing metal-organic framework compounds to a sample comprising chemical reactants and allowing the immobilized enzymes to catalyze a reaction between the reactants to form one or more reaction products.
Dependencies are inferred from the claim text.