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US 11,530,399 B2Granted
Hemicellulase compositions
AssigneeAjinomoto Co., Inc.
InventorsYusuke Hagiwara, Yasuhiro Mihara, Tomo Takagi, Satoshi Nakamura, Rie Yakushijin
Life of this patent
Estimating the term…
Abstract
Hemicellulase that degrades corn non-starch polysaccharides (“NSP”), DNA encoding the same, and a method of using the hemicellulase and its DNA are provided. Proteins having hemicellulase activity such as Xyn5A, Xyn10B, Xyn11A, Xyn30A, and Xyn43A are described.
Claims29
A composition comprising a first protein and a second protein, said first and second proteins are each selected from the group consisting of protein (A), protein (B), protein (C), and protein (D) provided that the composition comprises at least the protein (A) or (B);
- wherein the protein (A) is selected from the group consisting of:
- (A1) a protein comprising the amino acid sequence of positions 1 to 536 of SEQ ID NO:
- 26, positions 1 to 536 of SEQ ID NO:
- 28, or positions 1 to 536 of SEQ ID NO:
- 30, wherein said protein has hemicellulase activity;
- (A2) a protein comprising the amino acid sequence of positions 1 to 536 of SEQ ID NO:
- 26, positions 1 to 536 of SEQ ID NO:
- 28, or positions 1 to 536 of SEQ ID NO:
- 30, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said protein has hemicellulase activity;
- and
- (A3) a protein comprising an amino acid sequence showing an identity of 90% or higher to the amino acid sequence of positions 1 to 536 of SEQ ID NO:
- 26, positions 1 to 536 of SEQ ID NO:
- 28, or positions 1 to 536 of SEQ ID NO:
- 30, wherein said protein has hemicellulase activity;
- wherein the protein (B) is selected from the group consisting of:
- (B1) a protein comprising the amino acid sequence of positions 1 to 527 of SEQ ID NO:
- 12, or positions 1 to 390 of SEQ ID NO:
- 34, wherein said protein has hemicellulase activity,
- (B2) a protein comprising the amino acid sequence of positions 1 to 527 of SEQ ID NO:
- 12, or positions 1 to 390 of SEQ ID NO:
- 34, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said protein has hemicellulase activity, and
- (B3) a protein comprising an amino acid sequence showing an identity of 90% or higher to the amino acid sequence of positions 1 to 527 of SEQ ID NO:
- 12, or positions 1 to 390 of SEQ ID NO:
- 34, wherein said protein has hemicellulase activity;
- wherein the protein (C) is a hemicellulase of glucoside hydrolase family 10;
- and
- where in the protein (D) is a hemicellulose of glucoside hydrolase family 11.
24 more elements
A hemicellulase preparation comprising a protein selected from the group consisting of proteins (A), (B), and combinations thereof;
- wherein the protein (A) is selected from the group consisting of:
- (A1) a protein comprising the amino acid sequence of positions 1 to 536 of SEQ ID NO:
- 26, positions 1 to 536 of SEQ ID NO:
- 28, or positions 1 to 536 of SEQ ID NO:
- 30, wherein said protein has hemicellulase activity,
- (A2) a protein comprising the amino acid sequence of positions 1to to 536 of SEQ ID NO:
- 26, positions 1 to 536 of SEQ ID NO:
- 28, or positions 1 to 536 of SEQ ID NO:
- 30, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said protein has hemicellulase activity, and
- (A3) a protein comprising an amino acid sequence showing an identity of 90% or higher to the amino acid sequence of positions 1 to 536 of SEQ ID NO:
- 26, positions 1 to 536 of SEQ ID NO:
- 28, or positions 1 to 536 of SEQ ID NO:
- 30, wherein said protein has hemicellulase activity;
- wherein the protein (B) is selected from the group consisting of:
- (B1) a protein comprising the amino acid sequence of positions 1 to 527 of SEQ ID NO:
- 12, or positions 1 to 390 of SEQ ID NO:
- 34, wherein said protein has hemicellulase activity,
- (B2) a protein comprising the amino acid sequence of positions 1 to 527 of SEQ ID NO:
- 12, or positions 1 to 390 of SEQ ID NO:
- 34, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said protein has hemicellulase activity, and
- (B3) a protein comprising an amino acid sequence showing an identity of 90% or higher to the amino acid sequence of positions 1 to 527 of SEQ ID NO:
- 12, or positions 1 to 390 of SEQ ID NO:
- 34, wherein said protein has hemicellulase activity.
20 more elements
An animal feed additive comprising a protein selected from the group consisting of proteins (A), (B), and combinations thereof;
- wherein the protein (A) is selected from the group consisting of:
- (A1) a protein comprising the amino acid sequence of positions 1 to 536 of SEQ ID NO:
- 26, positions 1 to 536 of SEQ ID NO:
- 28, or positions 1 to 536 of SEQ ID NO:
- 30, wherein said protein has hemicellulase activity,
- (A2) a protein comprising the amino acid sequence of positions 1 to 536 of SEQ ID NO:
- 26, positions 1 to 536 of SEQ ID NO:
- 28, or positions 1 to 536 of SEQ ID NO:
- 30, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said protein has hemicellulase activity;
- and
- (A3) a protein comprising an amino acid sequence showing an identity of 90% or higher to the amino acid sequence of positions 1 to 536 of SEQ ID NO:
- 26, positions 1 to 536 of SEQ ID NO:
- 28, or positions 1 to 536 of SEQ ID NO:
- 30, wherein said protein has hemicellulase activity;
- wherein the protein (B) is selected from the group consisting of:
- (B1) a protein comprising the amino acid sequence of positions 1 to 527 of SEQ ID NO:
- 12, or positions 1 to 390 of SEQ ID NO:
- 34, wherein said protein has hemicellulase activity;
- (B2) a protein comprising the amino acid sequence of positions 1 to 527 of SEQ ID NO:
- 12, or positions 1 to 390 of SEQ ID NO:
- 34, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said protein has hemicellulase activity;
- and
- (B3) a protein comprising an amino acid sequence showing an identity of 90% or higher to the amino acid sequence of positions 1 to 527 of SEQ ID NO:
- 12, or positions 1 to 390 of SEQ ID NO:
- 34, wherein said protein has hemicellulase activity.
22 more elements
An animal feed comprising at least one protein
- selected from the group consisting of proteins (A), (B), and combinations thereof,
- wherein the protein (A) is selected from the group consisting of:
- (A1) a protein comprising the amino acid sequence of positions 1 to 536 of SEQ ID NO:
- 26, positions 1 to 536 of SEQ ID NO:
- 28, or positions 1 to 536 of SEQ ID NO:
- 30, wherein said protein has hemicellulase activity;
- (A2) a protein comprising the amino acid sequence of positions 1 to 536 of SEQ ID NO:
- 26, positions 1 to 536 of SEQ ID NO:
- 28, or positions 1 to 536 of SEQ ID NO:
- 30, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said protein has hemicellulase activity;
- and
- (A3) a protein comprising an amino acid sequence showing an identity of 90% or higher to the amino acid sequence of positions 1 to 536 of SEQ ID NO:
- 26, positions 1 to 536 of SEQ ID NO:
- 28, or positions 1 to 536 of SEQ ID NO:
- 30, wherein said protein has hemicellulase activity;
- wherein the protein (B) is selected from the group consisting of:
- (B1) a protein comprising the amino acid sequence of positions 1 to 527 of SEQ ID NO:
- 12, or positions 1 to 390 of SEQ ID NO:
- 34, wherein said protein has hemicellulase activity;
- (B2) a protein comprising the amino acid sequence of positions 1 to 527 of SEQ ID NO:
- 12, or positions 1 to 390 of SEQ ID NO:
- 34, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said protein has hemicellulase activity;
- and
- (B3) a protein comprising an amino acid sequence showing an identity of 90% or higher to the amino acid sequence of positions 1 to 527 of SEQ ID NO:
- 12, or positions 1 to 390 of SEQ ID NO:
- 34, wherein said protein has hemicellulase activity.
23 more elements
Dependencies are inferred from the claim text.