CRISPR-Cas effector polypeptides and methods of use thereof
AssigneeThe Regents of the University of California
InventorsJennifer A. Doudna, Basem Al-Shayeb, Jillian F. Banfield, Patrick Pausch
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Abstract
The present disclosure provides RNA-guided CRISPR-Cas effector proteins, nucleic acids encoding same, and compositions comprising same. The present disclosure provides ribonucleoprotein complexes comprising: an RNA-guided CRISPR-Cas effector protein of the present disclosure; and a guide RNA. The present disclosure provides methods of modifying a target nucleic acid, using an RNA-guided CRISPR-Cas effector protein of the present disclosure and a guide RNA. The present disclosure provides methods of modulating transcription of a target nucleic acid.
Claims26
A composition comprising:
- a) a polypeptide consisting of the amino acid sequence of SEQ ID NO:120;
- and
- b) a recombinant guide RNA comprising;
- i. a nucleotide sequence that is complementary to a target sequence of a target nucleic acid and
- ii. a region that associates with the polypeptide to form a guide RNA-polypeptide complex, wherein (i) is heterologous to (ii).
Dependencies are inferred from the claim text.