NADH-dependent enzyme mutants to convert acetone into isopropanol
AssigneeBraskem S.A.
InventorsVerônica Leite Queiroz, Lucas Pedersen Parizzi, Iuri Estrada Gouvea, Debora Noma Okamoto, Rafael Victório Carvalho Guido, Alessandro Silva Nascimento, Igor Polikarpov
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to biological processes and systems for the production of isopropanol and/or acetone utilizing modified alcohol dehydrogenases that exhibit increased activity with NADH as a cofactor. The disclosure further relates to polynucleotides and polypeptides of the modified alcohol dehydrogenases, and host cells containing the polynucleotides and expressing the polypeptides.
Claims14
A modified alcohol dehydrogenase that exhibits increased alcohol dehydrogenase activity with cofactor NADH as compared to an unmodified alcohol dehydrogenase of SEQ ID NO:
- 2, wherein the amino acid sequence of the modified alcohol dehydrogenase is one of SEQ ID NO:
- 3, 5, 6, 7, 8, 9, 10, 12, 13, 14, 15, 16, 18, 19, 20, 21, 22, 25, 26, 28, 29, 30, 31, 32, 33, 34, 35, 36, and 37.
A modified alcohol dehydrogenase, wherein the modified alcohol dehydrogenase exhibits alcohol dehydrogenase activity and has a cofactor preference for NADH over NADPH;
- wherein the modified alcohol dehydrogenase comprises the amino acid sequence of SEQ ID NO:
- 2 except for a combination of amino acid substitutions selected from:
- G198E and E247D;
- G198E and E247N;
- I173L and G198E;
- G198E, Y218F, and E247D;
- G198E and T248V;
- or
- G198E and Y218F;
- wherein the amino acid numbering corresponds to the amino acid sequence of SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.