Method for inducing differentiation into and producing beige and white adipocytes
AssigneeNissan Chemical Corporation
InventorTatsuro Kanaki
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Abstract
The present invention provides a method for producing a unilocular adipocyte including inducing differentiation into unilocular adipocytes of mesenchymal cells having differentiation potency into adipocytes by culturing the mesenchymal cells in suspension in a liquid medium composition capable of culturing cells or tissues in suspension, wherein the liquid medium composition contains a polymer compound having an anionic functional group that binds via a divalent metal cation to form a structure capable of suspending cells or tissues, and the method wherein the polymer compound is polysaccharide, preferably polysaccharide containing a glucuronic acid moiety, more preferably deacylated gellan gum, diutan gum or xanthan gum or a salt thereof.
Claims10
A method for producing a unilocular beige adipocyte comprising culturing mesenchymal cells having differentiation potential in a liquid medium composition, thereby inducing differentiation into the unilocular adipocyte,
- wherein
- the liquid medium composition comprises a polymer compound having an anionic functional group that binds via a divalent metal cation to form a structure capable of suspending the mesenchymal cells,
- the polymer compound is a polysaccharide,
- the mesenchymal cell is a precursor-adipocyte or a mesenchymal stem cell,
- the liquid medium composition comprises at least one adipocyte differentiation-inducing factor selected from the group consisting of insulin, glucocorticoid receptor agonist, cAMP phosphodiesterase inhibitor, cyclooxygenase inhibitor, prostaglandin, long chain fatty acid, triiodothyronine, and PPARγ agonist, and
- the unilocular adipocyte is UCP-1-positive.
Dependencies are inferred from the claim text.