Use of matrix cells for preparing a micro hair follicle
AssigneeL'oreal
InventorsKhalid Bakkar, Christine Collin-Djangone
Life of this patent
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Abstract
The invention relates to the use of matrix cells for obtaining a micro hair follicle and to the use thereof for evaluating the effect of cosmetic, pharmaceutical or dermatological products and also for the prophylactic or therapeutic treatment of a state of reduced pilosity.
Claims16
One or more micro hair follicles in the form of a bud that is obtained by means of an in vitro process comprising at least one step of culturing matrix cells which are located in the hair bulb in the presence of an effective amount of a ROCK inhibitor for a period of time sufficient to allow differentiation of said cells into keratinocytes positive for the K85 K35 markers, wherein the cells are keratinocytes positive for the K85 K35 markers and upon reaching confluence are in the form regular clusters and wherein the matrix cells are obtained from hair in the anagen phase and are free from other cell types, and wherein at least one bud comprises a structure in the form of keratinized fibre.
Dependencies are inferred from the claim text.