Anti-CD3 antibodies and methods of use
AssigneeGenentech, Inc.
InventorsXiaocheng Chen, Mark S. Dennis, Allen J. Ebens, Jr., Teemu T. Junttila, Robert F. Kelley, Mary A. Mathieu
Life of this patent
Estimating the term…
Abstract
The invention provides anti-cluster of differentiation 3 (CD3) antibodies and methods of using the same.
Claims20
One or more isolated nucleic acids encoding a bispecific antibody that binds to HER2 and CD3, wherein the bispecific antibody comprises:
- (a) an anti-HER2 arm comprising a first binding domain, the first binding domain comprising:
- a hypervariable region (HVR)-H1 comprising the amino acid sequence of SEQ ID NO:
- 169,
- an HVR-H2 comprising the amino acid sequence of SEQ ID NO:
- 170,
- an HVR-H3 comprising the amino acid sequence of SEQ ID NO:
- 171,
- an HVR-L1 comprising the amino acid sequence of SEQ ID NO:
- 172,
- an HVR-L2 comprising the amino acid sequence of SEQ ID NO:
- 173, and
- an HVR-L3 comprising the amino acid sequence of SEQ ID NO:
- 174;
- and
- (b) an anti-CD3 arm comprising a second binding domain, the second binding domain comprising:
- an HVR-H1 comprising the amino acid sequence of SEQ ID NO:
- 1,
- an HVR-H2 comprising the amino acid sequence of SEQ ID NO:
- 2,
- an HVR-H3 comprising the amino acid sequence of SEQ ID NO:
- 3,
- an HVR-L1 comprising the amino acid sequence of SEQ ID NO:
- 4,
- an HVR-L2 comprising the amino acid sequence of SEQ ID NO:
- 5, and
- an HVR-L3 comprising the amino acid sequence of SEQ ID NO:
- 6.
One or more isolated nucleic acids encoding a bispecific antibody that binds to HER2 and CD3, wherein the bispecific antibody comprises an anti-HER2 arm comprising a first binding domain comprising (a) a VH domain comprising the amino acid sequence of SEQ ID NO:
- 270 and (b) a VL domain comprising the amino acid sequence of SEQ ID NO:
- 271, and an anti-CD3 arm comprising a second binding domain comprising (a) a VH domain comprising the amino acid sequence of SEQ ID NO:
- 184 and (b) a VL domain comprising the amino acid sequence of SEQ ID NO:
- 185.
One or more isolated nucleic acids encoding a bispecific antibody that binds to HER2 and CD3, wherein the bispecific antibody comprises an anti-HER2 arm comprising a first binding domain comprising (a) a VH domain comprising the amino acid sequence of SEQ ID NO:
- 270 and (b) a VL domain comprising the amino acid sequence of SEQ ID NO:
- 271, and an anti-CD3 arm comprising a second binding domain comprising (a) a VH domain comprising the amino acid sequence of SEQ ID NO:
- 184 and (b) a VL domain comprising the amino acid sequence of SEQ ID NO:
- 185;
- and wherein:
- (a) the anti-CD3 arm further comprises a CH12 domain, a CH22 domain, and a CH32 domain, wherein the CH31 domain is paired with the CH32 domain;
- (b) the anti-HER2 arm further comprises a CH11 domain, a CH21 domain, a CH31 domain;
- and
- (c) the CH31 domain comprises a knob, the CH32 domain comprises a hole, and the knob is positionable in the hole.
Dependencies are inferred from the claim text.