Methods for purifying antibodies
AssigneeGlaxoSmithKline Intellectual Property Development Limited
InventorsAndre C. Dumetz, Kent E. Goklen, Nicholas E. Levy, Jessica Rachel Molek, Andrew S. Thomson, Kenneth G. Yancey
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Abstract
The present invention relates to a method of purifying a recombinant polypeptide from Host Cell Proteins (HCP), the method comprising: (a) applying a solution comprising the recombinant polypeptide and HCP to a superantigen chromatography solid support, (b) washing the superantigen chromatography solid support with a wash buffer comprising caprylate and arginine; and (c) eluting the recombinant polypeptide from the superantigen chromatography solid support.
Claims17
A method of purifying a recombinant polypeptide from Host Cell Proteins (HCP), the method comprising:
- (a) applying a solution comprising the recombinant polypeptide and HCP to a superantigen chromatography solid support, (b) washing the superantigen chromatography solid support with a wash buffer comprising at least about 75 mM caprylate and greater than about 0.5 M arginine;
- and (c) eluting the recombinant polypeptide from the superantigen chromatography solid support.
A method of purifying a recombinant polypeptide from phospholipase B-Like 2 protein, the method comprising:
- (a) applying a solution comprising the recombinant polypeptide and phospholipase B-Like 2 protein to a superantigen chromatography solid support, (b) washing the superantigen chromatography solid support with a wash buffer comprising about 100 mM caprylate and about 1.1 M arginine;
- and (c) eluting the recombinant polypeptide from the superantigen chromatography solid support.
A method of purifying a recombinant polypeptide from cathepsin L, the method comprising:
- (a) applying a solution comprising the recombinant polypeptide and cathepsin L to a superantigen chromatography solid support, (b) washing the superantigen chromatography solid support with a wash buffer comprising about 150 mM caprylate and about 1.1 M arginine;
- and (c) eluting the recombinant polypeptide from the superantigen chromatography solid support.
Dependencies are inferred from the claim text.