Back to results
US 11,529,587 B2Granted
Materials and methods for producing blood products
AssigneeCellphire, Inc.
InventorsJoshua Donald Montgomery, Braden Carl Ishler, Stephen Edward Amos, Keith Andrew Moskowitz, Amber Nicole Lee, Rafael Jorda, Glen Michael Fitzpatrick, Michael Alexander Mathews
Life of this patent
Estimating the term…
Abstract
Provided herein are materials and methods for the preparation of blood products. In one aspect, provided herein is a composition including platelets or platelet derivatives and an aqueous medium, wherein the aqueous medium has a protein concentration less than 50% of the protein concentration of donor apheresis plasma.
Claims29
A process for preparing a thermally-treated platelet composition, the process comprising:
- performing tangential flow filtration (TFF) of a platelet composition with a preparation agent comprising a buffering agent, trehalose in an amount in the range of 10 mM to 500 mM, and polysucrose in an amount in the range of 3% to 7%, thereby preparing a TFF-treated composition comprising at least 1000×103 platelets/μl in an aqueous medium having less than or equal to 7.5% plasma protein and having less than 5.0% microparticles by scattering intensity,
- freeze drying the TFF-treated composition comprising platelets in the aqueous medium to form a freeze-dried platelet composition;
- and
- heating the freeze-dried platelet composition at a temperature in the range of 60° C. to 85° C. for at least 1 hour to not more than 36 hours to thermally treat the freeze-dried platelets in the freeze-dried platelet composition to form the thermally-treated platelet composition, wherein thermally-treated platelets in the thermally-treated platelet composition have a potency of at least 1.5 thrombin generation potency units (TGPU) per 106 thermally-treated platelets.
1 more element
Dependencies are inferred from the claim text.