Engineered three-dimensional skin tissues, arrays thereof, and methods of making the same
AssigneesOrganovo, Inc., L'oreal
InventorsKelsey Nicole Retting, Colin M. O'Neill, Deborah Lynn Greene Nguyen, Sharon C. Presnell, Jessica Langer, Guive Balooch, Elizabeth Wu, Julien Demaude
Life of this patent
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Abstract
Disclosed are bioprinted, three-dimensional, biological skin tissues comprising: a dermal layer comprising dermal fibroblasts; and an epidermal layer comprising keratinocytes, the epidermal layer in contact with the dermal layer to form the three-dimensional, engineered, biological skin tissue. Also disclosed are arrays of engineered skin tissues and methods of making engineered skin tissues.
Claims18
A method of fabricating a three-dimensional, engineered, biological skin tissue, the method comprising:
- a. preparing a dermal bio-ink comprising dermal fibroblasts;
- b. preparing an epidermal bio-ink comprising keratinocytes;
- c. depositing the dermal bio-ink onto a surface, wherein the surface is not a scaffold;
- d. depositing the epidermal bio-ink such that the epidermal bio-ink contacts and forms a single layer on at least one surface of the dermal bio-ink;
- and
- e. maturing the deposited dermal and epidermal bio-inks in a cell culture media to allow the cells to cohere and differentiate to form the three-dimensional, engineered, biological skin tissue, wherein the three-dimensional, engineered, biological skin tissue comprises a plurality of layers of differentiated keratinocytes within 12 days of culture.
Dependencies are inferred from the claim text.