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US 11,529,424 B2Granted
Synthetic bioconjugates
AssigneeSymic Holdings, Inc.
InventorsJohn Eric Paderi, Julia Chen, Sharmistha Saha
Life of this patent
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Abstract
Provided herein are bioconjugates comprising a backbone and at least one branched or unbranched peptide having at least one collagen-binding unit covalently bonded thereto via a spacer and methods of use thereof.
Claims22
A bioconjugate comprising a glycan and from 1 to about 50 binding unit(s) of formula (I) covalently bonded thereto:
- ((X1)mX2)nX3-L (I)
- wherein:
- X1 is an amino acid sequence comprising a collagen-binding unit;
- X2 and X3 are independently absent, are an amino acid sequence having from 1 to 15 amino acids, or area moiety
- where p and q are each independently an integer from 1 to 10;
- L is a spacer of from 7 to 20 amino acids selected from the group consisting of glycine (G), serine (S), arginine (R), and lysine (K), provided L comprises at least two arginines (R) within the first five amino acids from the glycan, and wherein L further comprises an optional linking moiety which covalently bonds to the glycan;
- m is 1 or 2;
- and
- n is 1 or 2.
6 more elements
A bioconjugate comprising heparin conjugated to GQLYKSILYGSGRRGSG (SEQ ID NO:
- 95) in a 1:8 ratio or in a 1:5 ratio.
A binding unit of formula (II):
- ((X1)mX2)nX3-L (II)
- wherein:
- X1 is an amino acid sequence comprising a collagen-binding unit comprising from 3 to about 10 amino acids;
- X2 and X3 are independently absent, are an amino acid sequence having from 1 to 15 amino acids, or area moiety
- where p and q are each independently an integer from 1 to 10;
- L is a spacer of from 7 to 20 amino acids selected from the group consisting of glycine (G), serine (S), arginine (R), and lysine (K), provided L comprises at least two arginines (R) within the first five amino acids from the terminus, and wherein L further comprises an optional linking moiety;
- m is 1 or 2;
- and
- n is 1 or 2;
- provided that the binding unit is not RRRKKIQGRSKR (SEQ ID NO:
- 2) or RRGGRKWGSFEG (SEQ ID NO:
- 3).
9 more elements
A peptide having the amino acid sequence RRANAALKAGELYKSILYGSGSGSRR (SEQ ID NO:
- 128), RRANAALKAGELYKSILYGSGSGSRR—NHNH2 (SEQ ID NO:
- 128), RRANAALKAGQLYKSILYGSGSGSRR (SEQ ID NO:
- 6), RRANAALKAGQLYKSILYGSGSGSRR—NHNH2 (SEQ ID NO:
- 6), (GQLYKSILYGSG)2-KSGSRR (SEQ ID NO:
- 59 and SEQ ID NO:
- 130), or (GQLYKSILYGSG)2-KSGSRR—NHNH2 (SEQ ID NO:
- 59 and SEQ ID NO:
- 130).
5 more elements
Dependencies are inferred from the claim text.