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US 11,529,421 B2Granted
Modified globin proteins
AssigneeUniversity of Essex Enterprises Limited
InventorsChris E. Cooper, Brandon Reeder, Gary Silkstone
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Abstract
Certain aspects of the present invention relate to modified hemoglobin proteins comprising at least one modification (e.g., at the thiol group of an exogenous amino acid residue) for homogenous conjugation of one or more polymeric moieties e.g. polyethylene glycol or derivatives thereof. Also included herein are methods of using such modified proteins and compositions comprising such proteins, e.g. in therapy.
Claims19
A conjugate comprising:
- (a) at least one polymeric moiety comprising a polyethylene glycol molecule (PEG) or a polyalkylene glycol (PAG) and/or having a molecular weight of about 20,000 Daltons (20 kDa);
- and
- (b) at least one recombinant modified hemoglobin chain subunit comprising at least one alpha chain subunit;
- wherein endogenous amino acid residue alanine 19 of the alpha chain subunit is substituted with at least one exogenous amino acid residue comprising at least one reactive thiol group for conjugation to the at least one polymeric moiety;
- wherein the at least one polymeric moiety is conjugated to the at least one exogenous amino acid residue;
- and
- wherein, if the conjugate further comprises at least one additional recombinant modified hemoglobin chain subunit comprising a beta and/or gamma chain subunit, endogenous amino acid residue cysteine 93 of the beta and/or gamma chain subunit is deleted or substituted with an amino acid residue which does not comprise a reactive thiol group.
4 more elements
Dependencies are inferred from the claim text.