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US 11,529,411 B2Granted
Polynucleotide encoding trimeric S1-CD40L fusion protein immunogen against middle east respiratory syndrome-corona virus
AssigneeKing Abdulaziz University
InventorAnwar M. Hashem
Life of this patent
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Abstract
An immunogenic CD40-targeted trimeric MERS-CoV S1 fusion polypeptide as well as a corresponding polynucleotide encoding it and its use for safely inducing immune responses directed against MERS-CoV without inducing vaccine associated respiratory pathologies associated with non-targeted vaccines.
Claims18
A polynucleotide encoding a fusion protein comprising:
- at least one immunogenic fragment, receptor-binding domain, or epitope of S1 polypeptide of Middle East respiratory syndrome coronavirus (MERS-CoV),
- a trimerization motif, and
- a ligand that binds to CD40;
- and optionally,
- one or more linkers between the at least one immunogenic fragment, receptor-binding domain, or epitope of S1 polypeptide, the trimerization motif, and the ligand that binds to CD40,
- wherein the fusion protein comprises an S1 polypeptide that has an amino acid sequence that is at least 70% identical to SEQ ID NO:
- 2;
- the trimerization motif comprises SEQ ID NO:
- 6 and the ligand that binds to CD40 is at least 70% identical to the amino acid sequence of SEQ ID NO:
- 23 or SEQ ID NO:
- 25 or 27 or to a CD40-binding fragment thereof;
- and the peptide linker, when present, comprises SEQ ID NO:
- 8.
10 more elements
A polynucleotide encoding a fusion protein comprising:
- at least one immunogenic fragment, receptor-binding domain, or epitope of S1 polypeptide of Middle East respiratory syndrome coronavirus (MERS-CoV),
- a trimerization motif, and
- a ligand that binds to CD40;
- and optionally,
- one or more linkers between the at least one immunogenic fragment, receptor-binding domain, or epitope of S1 polypeptide, the trimerization motif, and the ligand that binds to CD40,
- wherein the fusion protein comprises at least one trimerization motif comprising the amino acid sequence of SEQ ID NO:
- 6.
4 more elements
A nucleic acid based vaccine comprising a polynucleotide encoding a fusion protein comprising:
- at least one immunogenic fragment, receptor-binding domain, or epitope of S1 polypeptide of Middle East respiratory syndrome coronavirus (MERS-CoV),
- a trimerization motif, and
- a ligand that binds to CD40;
- and optionally,
- one or more linkers between the at least one immunogenic fragment, receptor-binding domain, or epitope of S1 polypeptide, the trimerization motif, and the ligand that binds to CD40, or
- a vector or host cell containing said polynucleotide, and a pharmaceutically acceptable carrier, excipient or adjuvant;
- wherein the nucleic acid based vaccine further comprises a hybrid S protein segment expressing S protein epitopes from more than one strain of MERS-CoV or which comprises a consensus S protein segment expressing S protein epitopes from more than one strain of MERS-CoV.
4 more elements
Dependencies are inferred from the claim text.